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Development of a novel dual priming oligonucleotide system-based PCR assay for specific detection ofSalmonellafrom food samples

文献类型： 外文期刊

作者： Li, Dan-Dan 1 ; Hao, Chun-Bo 2 ; Liu, Zhong-Mei 3 ; Wang, Sui-Jia 1 ; Wang, Yu 4 ; Chao, Zhe 5 ; Gao, Shen-Yang 6 ; Chen, 1 ;

作者机构： 1.Haikou Customs Dist PR China, Haikou, Hainan, Peoples R China

2.Hainan Prov Peoples Hosp, Haikou, Hainan, Peoples R China

3.Harbin Customs Dist PR China, Harbin, Peoples R China

4.Chongqing Customs Dist PR China, Chongqing, Peoples R China

5.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou, Hainan, Peoples R China

6.Jinzhou Med Univ, Jinzhou, Peoples R China

7.Hainan Univ, Coll Anim Sci & Technol, Haikou, Hainan, Peoples R China

期刊名称：JOURNAL OF FOOD SAFETY （ 影响因子：1.953； 五年影响因子：1.946 ）

ISSN： 0149-6085

年卷期： 2020 年 40 卷 3 期

页码：

收录情况： SCI

摘要： In this study, a novel dual priming oligonucleotide (DPO) system-based polymerase chain reaction (PCR; DPO system-based PCR) assay, which detected thefimYgene ofSalmonella, was developed for the fast food testing. The DPO system-based PCR assay allowed a wide range of annealing temperatures at 48-68 degrees C to efficiently amplifyfimYgene with an analytical detection limit of 1.2 x 10(2)CFU/ml forSalmonellain pure cultures and artificially contaminated food matrix. Significantly, the presence of a bubble-like polydeoxyinosine (polyI) linker in the DPO system brought an unparalleled high specificity in the identification of target bacteria, and consequently, the false positives and mismatches of PCR process can be eliminated in priming. Applying the DPO system-based PCR assay to 285 collected food samples revealed that 29 samples were positive in this assay, in accordance with the results of conventional culture-based method, indicating a potential diagnostic capability. The high specificity of the DPO system-based PCR indicates its great potential to be a quick, reliable and practical method for the detection ofSalmonellain foods.

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