Expression profile and estrogenic regulation of Amh during gonadal sex differentiation in northern snakehead (Channa argus)
文献类型： 外文期刊
作者： Luo, Qing 1 ; Ou, Mi 1 ; Zhao, Jian 1 ; Liu, Haiyang 1 ; Gao, Dandan 1 ; Wu, Yanduo 1 ; Zhang, Lanlan 3 ; Chen, Kunci 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Trop & Subtrop Fishery Resources Applicat, Guangzhou 510380, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Sun Yat Sen Univ, Zhongshan Sch Med, 74 Zhongshan 2nd Rd, Guangzhou 510080, Peoples R China
关键词： Channa argus; Amh; Estrogen; Sex differentiation
期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）
ISSN： 1976-9571
年卷期： 2020 年 42 卷 7 期
页码：
收录情况： SCI
摘要： Background Anti-Mullerian hormone (Amh) plays a critical role in both early sex determination and later gonad development in vertebrate species. However, it remains unknown in northern snakehead (Channa argus), which is economically important freshwater fish with sexual dimorphism. Objective This study aimed to identify the expression profiles and estrogenic regulation ofCaAmhduring gonadal sex differentiation inC. argus. Methods The cDNA and genomic DNA sequences ofCaAmhwere identified by PCR and RACE techniques. The expression patterns ofCaAmhwere detected by qRT-PCR during the gonadal sex differentiation and after 17 alpha-ethinyloestradiol (EE2) treatments. Results CaAmhis composed of seven exons and six introns, and its full-length cDNA is 2413 bp in length, with 1635 bp open reading frame (ORF) that encodes a 544 amino acid protein. Tissues expression patterns revealed thatCaAmhdisplay the highest expression in testis of XY males (40.36 folds,p < 0.01). The spatio-temporal expression patterns during gonadal sex differentiation indicated thatCaAmhexpression differed between XX females and XY males at 30 day after hatching (dah), and reached to the peak (36.03 folds,p < 0.01) at 90 dah in XY gonads. However,CaAmhexpression in XX gonads remained low throughout the sampling period. Furthermore,CaAmhexpression in the gonads (ovaries) of the sex-reversed XY fish (XY-F) by the administration of estrogen EE2 was downregulated to low level, similar to that in ovaries of normal XX females (XX-F). Conclusions These results show that Amh plays a critical role in testicular differentiation ofC. argusand it is apparently modulated by estrogens in this species.
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