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Direct regeneration of haploid or doubled haploid plantlets in cucumber (Cucumis sativusL.) through ovary culture

文献类型： 外文期刊

作者： Deng, Ying 1 ; Tang, Bing 2 ; Zhou, Xia 1 ; Fu, Wenyuan 2 ; Tao, Lian 2 ; Zhang, Lu 1 ; Chen, Jinfeng 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Guizhou Acad Agr Sci, Inst Hort, Guiyang 55006, Peoples R China

关键词： Cucumber (cucumis sativusL; ); Ovary culture; Doubled haploid; Direct regeneration

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2020 年 142 卷 2 期

页码：

收录情况： SCI

摘要： This paper reports a simple and effective method of directly producing cucumber plants through unfertilized ovary culture. The paper involves an experiment investigating factorial interactions between TDZ treatment, cold pretreatment, genotypes of cucumber, to improve plant induction. Cold pretreatment was effective in stimulating the ovary. The results showed that cold pretreatment for 4 days, TDZ for 0.06 mg center dot L-1, the interaction with genotype can be used as an effective strategy to improve the efficiency of gynogenesis. The plant regeneration induction rate was highest (79.3%). In addition, we observed the process of cucumber megasporogenesis and plant regeneration. The plants obtained from ovary culture of cucumber were identified as diploid or haploid by flow cytometry, consistent with the results of chromosome counting. The diploid plants were further identified as pure doubled haploid using simple sequence repeats (SSR). The doubling treatment we used was one of the simplest and most effective methods, completed in a short time (1 h) with a doubling rate of 75%. The acclimation rate for the surviving was 70%. This work provides a basis for promoting haploid breeding in cucumber.

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