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Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

文献类型： 外文期刊

作者： Zhu, De 1 ; Li, Ximei 1 ; Wang, Zhiwei 1 ; You, Chunyuan 4 ; Nie, Xinhui 5 ; Sun, Jie 5 ; Zhang, Xianlong 1 ; Zhang, Dawe 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Qingdao Agr Univ, Coll Agron, Shandong Key Lab Dryland Farming Technol, Shandong Engn Res Ctr Germplasm Innovat Utilizat, Qingdao 266109, Shandong, Peoples R China

3.Shandong Peanut Res Inst, Qingdao 266109, Shangdong, Peoples R China

4.Shihezi Acad Agr Sci, Cotton Res Inst, Shihezi 832003, Xinjiang, Peoples R China

5.Shihezi Univ, Key Lab Oasis Ecol Agr Xinjiang Bingtuan, Agr Coll, Shihezi 832003, Xinjiang, Peoples R China

6.Xinjiang Acad Agr Sci, Inst Ind Crops, Urumqi 830091, Xinjiang, Peoples R China

关键词： Cotton; Chromosome substituted segments lines (CSSLs); Quantitative trait loci (QTL); Whole genome re-sequencing; Cottonseed oil content (SOC)

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTL) in interspecific hybridization. Results In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing ofGossypium hirsutum(Gh) andG. barbadense(Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.

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