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Single-cell resolution analysis reveals the preparation for reprogramming the fate of stem cell niche in cotton lateral meristem

文献类型： 外文期刊

作者： Zhu, Xiangqian 1 ; Xu, Zhongping 1 ; Wang, Guanying 1 ; Cong, Yulong 1 ; Yu, Lu 1 ; Jia, Ruoyu 1 ; Qin, Yuan 1 ; Zhang, Guangyu 1 ; Li, Bo 2 ; Yuan, Daojun 1 ; Tu, Lili 1 ; Yang, Xiyan 1 ; Lindsey, Keith 3 ; Zhang, Xianlong 1 ; Jin, Shuangxia 1 ;

作者机构： 1.Huazhong Agr Univ, Hubei Hongshan Lab, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Xinjiang Key Lab Crop Biotechnol, Xinjiang 830000, Peoples R China

3.Univ Durham, Dept Biosci, Durham DH1 3Le, England

关键词： Cotton; Plant regeneration; scRNA-seq; Gene regulatory network; Gene functional verification

期刊名称：GENOME BIOLOGY （ 影响因子：12.3； 五年影响因子：17.4 ）

ISSN： 1474-760X

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Background Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible for cell reprogramming during somatic embryogenesis are still largely unclear. Recent advances in single-cell technologies enable us to explore the mechanism of plant regeneration at single-cell resolution.Results We generate a high-resolution single-cell transcriptomic landscape of hypocotyl tissue from the highly regenerable cotton genotype Jin668 and the recalcitrant TM-1. We identify nine putative cell clusters and 23 cluster-specific marker genes for both cultivars. We find that the primary vascular cell is the major cell type that undergoes cell fate transition in response to external stimulation. Further developmental trajectory and gene regulatory network analysis of these cell clusters reveals that a total of 41 hormone response-related genes, including LAX2, LAX1, and LOX3, exhibit different expression patterns in the primary xylem and cambium region of Jin668 and TM-1. We also identify novel genes, including CSEF, PIS1, AFB2, ATHB2, PLC2, and PLT3, that are involved in regeneration. We demonstrate that LAX2, LAX1 and LOX3 play important roles in callus proliferation and plant regeneration by CRISPR/Cas9 editing and overexpression assay.Conclusions This study provides novel insights on the role of the regulatory network in cell fate transition and reprogramming during plant regeneration driven by somatic embryogenesis.

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