文献类型： 外文期刊
作者： Tang, Biao 1 ; Tang, Yizhi 3 ; Zhang, Ling 1 ; Liu, Xiao 6 ; Chang, Jiang 1 ; Xia, Xiaodong 5 ; Yang, Hua 1 ; Shen, Zhang 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Hangzhou, Peoples R China
3.Sichuan Univ, Minist Educ, Key Lab Bioresource & Ecoenvironm, Anim Dis Prevent, Chengdu, Peoples R China
4.Sichuan Univ, Coll Life Sci, Food Safety Key Lab Sichuan Prov, Chengdu, Peoples R China
5.Northwest Agr & Forestry Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China
6.China Agr Univ, Coll Vet Med, Beijing Lab Food Qual & Safety, Beijing Key Lab Detect Technol Anim Derived Food, Beijing, Peoples R China
关键词： Campylobacter; fexA; multidrug resistance; food safety
期刊名称：ANTIMICROBIAL AGENTS AND CHEMOTHERAPY （ 影响因子：5.191； 五年影响因子：5.346 ）
ISSN： 0066-4804
年卷期： 2020 年 64 卷 7 期
页码：
收录情况： SCI
摘要： Florfenicol belongs to a class of phenicol antimicrobials widely used as feed additives and for the treatment of respiratory infections. In recent years, increasing resistance to florfenicol has been reported in Campylobacter spp., the leading foodborne enteric pathogens causing diarrheal diseases worldwide. Here, we reported the identification of fexA, a novel mobile florfenicol resistance gene in Campylobacter. Of the 100 Campylobacter jejuni strains isolated from poultry in Zhejiang, China, 9 were shown to be fexA positive, and their whole-genome sequences were further determined by integration of Illumina short-read and MinION long-read sequencing. The fexA gene was found in the plasmid of one strain and chromosomes of eight strains, and its location was verified by S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting. Based on comparative analysis, the fexA gene was located within a region with the tet(L)-fexA-catA-tet(O) gene arrangement, demonstrated to be successfully transferable among C. jejuni strains. Functional cloning indicated that acquisition of the single fexA gene significantly increased resistance to florfenicol, whereas its inactivation resulted in increased susceptibility to florfenicol in Campylobacter. Taken together, these results indicated that the emerging fexA resistance is horizontally transferable, which might greatly facilitate the adaptation of Campylobacter in food production environments where florfenicols are frequently used.
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