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Pseudorabies virus (PRV) strain with defects in gE, gC, and TK genes protects piglets against an emerging PRV variant

文献类型： 外文期刊

作者： Lin, Jinxin 1 ; Li, Zhaolong 2 ; Feng, Zhihua 1 ; Fang, Zhou 1 ; Chen, Jianghua 1 ; Chen, Wengzhi 1 ; Liang, Wangwang; 1 ;

作者机构： 1.Fujian Normal Univ, Fujian Key Lab Innate Immune Biol, Qishan Campus,8 Xuefu South Rd, Fuzhou 350117, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, 100 Pudang Rd, Fuzhou 350013, Fujian, Peoples R China

关键词： gC/gE/TK deletion; PRV-Delta gaC\gE Delta TK; pseudorabies virus; vaccine

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2020 年 82 卷 6 期

页码：

收录情况： SCI

摘要： The prevalence of an emerging variant of the pseudorabies virus (PRV) has been causing serious losses to farmers in China. Moreover, the commercially available PRV vaccine often fails to provide thorough protection. Therefore, in this study, we generated a PRV-Delta gaC\gE Delta TK strain with defects in gC, gE, and TK of PRV. Compared to the parental PRV strain and the single gene deletion strains (PRV-Delta gC, PRV-Delta gE, and PRV-Delta TK), PRV-Delta gC\gE Delta TK grew slowly, and exhibited fewer and smaller plaques on swine testis (ST) cells. Furthermore, animal experiment results showed that mice that were immunized intramuscularly with PRV-Delta gC\gE Delta TK, survived throughout the experiment with no observed clinical symptoms, and were completely protected against PRV challenge. Additionally, deletion of the gC, gE, and TK genes significantly alleviated viral damage in the brain. Furthermore, one-day-old weaned piglets immunized intramuscularly with PRV-Delta gC\gEATK elicited higher levels of gB antibodies against both the emerging PRV variant and the parental PRV, exhibited full protection against challenge with both variants, and showed neutralization capacity against PRV. These data suggest that PRV-Delta gC\gE Delta TK is a promising vaccine candidate for the control of pseudorabies.

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