The Disruption of an OxyR-Like Protein Impairs Intracellular Magnetite Biomineralization in Magnetospirillum gryphiswaldense MSR-1
文献类型： 外文期刊
作者： Zhang, Yunpeng 1 ; Wen, Tong 1 ; Guo, Fangfang 1 ; Geng, Yuanyuan 1 ; Liu, Junquan 1 ; Peng, Tao 1 ; Guan, Guohua 1 ; Tia 1 ;
作者机构： 1.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing, Peoples R China
2.China Agr Univ, Coll Biol Sci, Minist Agr, Key Lab Soil Microbiol, Beijing, Peoples R China
3.France China Biomineralizat & Nanostruct Lab, Beijing, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Live, Beijing, Peoples R China
5.Peking Univ, Coll Chem & Mol Engn, Beijing, Peoples R China
关键词： OxyR-like protein; magnetosome formation; Magnetospirillum gryphiswaldense MSR-1; carbon metabolism; biomineralization
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2017 年 8 卷
页码：
收录情况： SCI
摘要： Magnetotactic bacteria synthesize intracellular membrane-enveloped magnetite bodies known as magnetosomes which have been applied in biotechnology and medicine. A series of proteins involved in ferric ion transport and redox required for magnetite formation have been identified but the knowledge of magnetosome biomineralization remains very limited. Here, we identify a novel OxyR homolog (named OxyR-Like), the disruption of which resulted in low ferromagnetism and disfigured nano-sized iron oxide crystals. High resolution-transmission electron microscopy showed that these nanoparticles are mainly composed of magnetite accompanied with ferric oxide including a-Fe2O3 and +-Fe2O3. Electrophoretic mobility shift assay and DNase I footprinting showed that OxyR-Like binds the conserved 5'-GATA-N{9}-TATC-3' region within the promoter of pyruvate dehydrogenase (pdh) complex operon. Quantitative real-time reverse transcriptase PCR indicated that not only the expression of pdh operon but also genes related to magnetosomes biosynthesis and tricarboxylic acid cycle decreased dramatically, suggesting a link between carbon metabolism and magnetosome formation. Taken together, our results show that OxyR-Like plays a key role in magnetosomes formation.
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