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iTRAQ-based comparative proteomic analysis reveals high temperature accelerated leaf senescence of tobacco (Nicotiana tabacum L.) during flue-curing

文献类型： 外文期刊

作者： Wu, Shengjiang 1 ; Guo, Yushuang 2 ; Joan, Heren Issaka 4 ; Tu, Yonggao 2 ; Adil, Muhammad Faheem 4 ; Sehar, Shafaq 1 ;

作者机构： 1.Guizhou Univ, State Key Lab Breeding Base Green Pesticide & Agr, Key Lab Plant Resources Conservat & Germplasm Inn, Minist Educ, Guiyang 550025, Peoples R China

2.Guizhou Acad Tobacco Sci, Key Lab Mol Genet, Upland Flue Cured Tobacco Qual & Ecol Key Lab, CNTC, Guiyang 550081, Peoples R China

3.Guizhou Acad Agr Sci, Guiyang 550006, Peoples R China

4.Zhejiang Univ, Dept Agron, Coll Agr & Biotechnol, Key Lab Crop Germplasm Resource, Hangzhou 310058, Peoples R China

关键词： Tobacco; Postharvest physiology; Curing; qRT-PCR; iTRAQ

期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）

ISSN： 0888-7543

年卷期： 2020 年 112 卷 5 期

页码：

收录情况： SCI

摘要： Tobacco (Nicotiana tabacum ) is extensively cultivated all over the world for its economic value. During curing and storage, senescence occurs, which is associated with physiological and biochemical changes in postharvest plant organs. However, the molecular mechanisms involved in accelerated senescence due to high temperatures in tobacco leaves during curing need further elaboration. We studied molecular mechanisms of senescence in tobacco leaves exposed to high temperature during curing (Fresh, 38 degrees C and 42 degrees C), revealed by isobaric tags for relative and absolute quantification (iTRAQ) for the proteomic profiles of cultivar Bi'na1. In total, 8903 proteins were identified, and 2034 (1150 up-regulated and 1074 down-regulated) differentially abundant proteins (DAPs) were obtained from tobacco leaf samples. These DAPs were mainly involved in posttranslational mod- ification, protein turnover, energy production and conversion. Sugar- and energy-related metabolic biological processes and pathways might be critical regulators of tobacco leaves exposed to high temperature during senescence. High-temperature stress accelerated tobacco leaf senescence mainly by down-regulating photo-synthesis-related pathways and degrading cellular constituents to maintain cell viability and nutrient recycling. Our findings provide a valuable inventory of novel proteins involved in senescence physiology and elucidate the protein regulatory network in postharvest organs exposed to high temperatures during flue-curing.

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