MAT1-1-3, a Mating Type Gene in theVillosiclava virens, Is Required for Fruiting Bodies and Sclerotia Formation, Asexual Development and Pathogenicity
文献类型： 外文期刊
作者： Yong, Mingli 1 ; Yu, Junjie 1 ; Pan, Xiayan 1 ; Yu, Mina 1 ; Cao, Huijuan 1 ; Qi, Zhongqiang 1 ; Du, Yan 1 ; Zhang, Rongs 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
关键词： mating type gene; Villosiclava virens; MAT1-1-3; sexual development; pathogenicity
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Villosiclava virensis the prevalent causative pathogen of rice false smut, a destructive rice disease. Mating-type genes play a vital role in the evolution of mating systems in fungi. Some fungi have lostMAT1-1-3, one of the mating-type genes, during evolution, whereas others still retainMAT1-1-3. However, howMAT1-1-3regulates the sexual development of heterothallicV. virensremains unknown. Here, we generated theMAT1-1-3mutants, which exhibited defects in vegetative growth, stress response, pathogenicity, sclerotia formation and fruiting body maturation. An artificial outcrossing inoculation assay showed that the Delta mat1-1-3mutant was unable to produce sclerotia. Unexpectedly, the Delta mat1-1-3mutant could form immature fruiting bodies without mating on potato sucrose agar medium (PSA) compared with the wild-type strain, most likely by activating the truncatedMAT1-2-1transcription to regulate the sexual development. Moreover, RNA-seq data showed that knockout ofMAT1-1-3results in misregulation of a subset of genes involved in sexual development, MAPK signaling, cell wall integrity, autophagy, epigenetic modification, and transcriptional regulation. Collectively, this study reveals thatMAT1-1-3is required for asexual and sexual development, and pathogenicity ofV. virens, thereby provides new insights into the function of mating-type genes in the fungi life cycle and infection process.
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