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Investigation of genes associated with petal variations between diploid and autotetraploid in Chinese cabbage (Brassica rapaL. ssp.pekinensis) by RNA-seq and sRNA-seq

文献类型： 外文期刊

作者： Shi, Fengyan 1 ; Wang, Yiheng 2 ; Huang, Shengnan 1 ; Dong, Shiyao 1 ; Liu, Zhiyong 1 ; Feng, Hui 1 ;

作者机构： 1.Shenyang Agr Univ, Dept Hort, 120 Dongling Rd, Shenyang 110866, Peoples R China

2.Tianjin Acad Agr Sci, Jinjing Rd 17 Km, Tianjin 300384, Peoples R China

关键词： Autotetraploid; Chinese cabbage; Petal; Transcriptome; miRNA

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN： 1617-4615

年卷期： 2020 年 295 卷 6 期

页码：

收录情况： SCI

摘要： Polyploidy promotes morphological, physiological, and reproductive diversity in plants. The imminent effect of chromosome doubling in plants is the enlargement of organs such as flowers and fruits, which increases the commercial value of crops. Flowering plays a vital role in the growth and development of angiosperms. Here, we prepared an isolated microspore culture of 'FT', a doubled haploid (DH) line of Chinese cabbage (Brassica rapaL. ssp.pekinensis), and obtained diploid and autotetraploid plants with the same genetic background. Compared with diploids, the autotetraploids were characterized by large floral organs, dark petals, delayed flowering, and reduced fertility. The indole-3-acetic acid (IAA) and jasmonic acid (JA) levels in autotetraploid petals were significantly higher and the abscisic acid (ABA) level was significantly lower than those in the diploid petals. The lutein level in autotetraploid petals was nearly two times higher than that in the diploid petals. A comparative transcriptome analysis revealed 14,412 differentially expressed genes (DEGs) between the diploids and autotetraploids, and they were enriched in 117 Gene Ontology terms and 110 Kyoto Encyclopedia of Genes and Genomes pathways. We detected 231 DEGs related to phytohormone signal transduction and 29 DEGs involved in carotenoid biosynthesis. An miRNA-target mRNA analysis showed that 32 DEGs regulated by 16 DEMs were associated with flowering timing (BraA03000336,BraA09004319, andBraA09000515), petal development (BraA05002408,BraA01004006,BraA09004069, andBraA04000966), flower opening (BraA07000350), and pollen development (BraA01000720,BraA09005727, andBraA01000253). This study provides information to help elucidate the molecular mechanisms underlying phenotypic variations induced by autopolyploidy in Chinese cabbage.

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