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Knocking Out the Transcription Factor OsNAC092 Promoted Rice Drought Tolerance

文献类型： 外文期刊

作者： Wang, Bo 1 ; Wang, Yiheng 1 ; Yu, Wancong 1 ; Wang, Luping 1 ; Lan, Qingkuo 1 ; Wang, Yong 1 ; Chen, Chengbin 2 ; Zhang, Yong 3 ;

作者机构： 1.Tianjin Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Tianjin 300071, Peoples R China

2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

3.Univ Elect Sci & Technol China, Ctr Informat Biol, Sch Life Sci & Technol, Dept Biotechnol, Chengdu 610054, Peoples R China

关键词： rice; NAC transcription factor; CRISPR-Cas9; drought stress; transcriptome analysis

期刊名称：BIOLOGY-BASEL （ 影响因子：5.168； ）

ISSN：

年卷期： 2022 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Environmental drought stress threatens rice production. Previous studies have reported that related NAC (NAM, ATAF1/2, and CUC) transcription factors play an important role in drought stress. Herein, we identified and characterized OsNAC092, encoding an NAC transcription factor that is highly expressed and induced during drought tolerance. OsNAC092 knockout lines created using the clustered regularly interspaced palindromic repeats (CRISPR)-associated protein 9 (Cas9) system exhibited increased drought resistance in rice. RNA sequencing showed that the knockout of OsNAC092 caused a global expression change, and differential gene expression is chiefly associated with "response to light stimulus," "MAPK signaling pathway," "plant hormone signal transduction," "response to oxidative stress," "photosynthesis," and "water deprivation." In addition, the antioxidants and enzyme activities of the redox response were significantly increased. OsNAC092 mutant rice exhibited a higher ability to scavenge more ROS and maintained a high GSH/GSSG ratio and redox level under drought stress, which could protect cells from oxidant stress, revealing the importance of OsNAC092 in the rice's response to abiotic stress. Functional analysis of OsNAC092 will be useful to explore many rice resistance genes in molecular breeding to aid in the development of modern agriculture.

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