Overexpression of Cotton Laccase Gene LAC1 Enhances Resistance to Botrytis cinereal in Tomato Plants
文献类型： 外文期刊
作者： Liu, Jia-Jia 1 ; Zhuang, Yu 1 ; Huang, Xiao-Zhen 1 ; Zhao, De-Gang 1 ; Zhao, Yi-Chen 1 ;
作者机构： 1.Guizhou Univ, Minist Educ, Key Lab Plant Resources Conservat & Germplasm Inn, Coll Tea Sci,Coll Life Sci, Guiyang 550025, Peoples R China
2.Guizhou Acad Agr Sci, Guiyang 550006, Peoples R China
关键词： GhLAC1; Laccase; Transgenic tomato; Lignin; Gray mold
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2020 年 24 卷 2 期
页码：
收录情况： SCI
摘要： Laccase is a glycoprotein oxidase containing four copper ions found in bacteria, fungi, insects and plants. In higher plants, laccase is mainly involved in the synthesis of lignin and the regulation of the content of phenolic substances in plants to enhance the plant's defense against diseases and pests. In this study, we constructed an overexpression vector harboring the Gossypium hirsutum LAC1 gene and introduced it into tomato (Solanum lycopersicum L.). The laccase activity, gray mold resistance and lignin level in transgenic tomato overexpressing GhLAC1 were analyzed using the wild-type as the control. The former had significantly higher laccase activity and gray-mold resistance levels compared with those of wild-type. Additionally, compared with the control plants, the lignin contents in the leaves and stems of the transgenic tomato were significantly higher. Scanning electron microscopy was used to observe the cross sections of wild-type and transgenic tomato stem segments and the cell wall near the xylem catheter of the latter was significantly thicker than that of the former and the outline was more distinct. Thus, the GhLAC1 gene significantly increased the lignin content in tomato, thickened the cell walls and strengthened physical defenses, thereby increasing tomato resistance to gray mold. (C) 2020 Friends Science Publishers
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