Development of High-Density Genetic Map by Specific-Locus Amplified Fragment (SLAF) Sequencing and Identification of QTLs Governing Flowering and Bolting Time in Chinese Kale
文献类型： 外文期刊
作者： Li, Guihua 1 ; Guo, Juxian 1 ; Luo, Wenlong 1 ; Anjum, Tehmina 2 ; Akram, Waheed 1 ; Ahmad, Aqeel 1 ; Ali, Basharat 3 ; A 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Res Vegetables, Vegetable Res Inst, Guangzhou, Peoples R China
2.Univ Punjab, Inst Agr Sci, Lahore, Pakistan
3.Univ Punjab, Dept Microbiol & Mol Genet, Lahore, Pakistan
4.Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou, Peoples R China
关键词： Bolting time; Chinese kale; Flowering time; Genetic map; SLAF-seq
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2020 年 24 卷 3 期
页码：
收录情况： SCI
摘要： This study was aimed on finding quantitative trait loci (QTL) controlling flowering (FT) and bolting (BT) time traits in Chinese kale (Brassica oleracea var. Alboglabra) along with the candidate gene analysis. Firstly, a genetic map of high density was created by Specific-locus amplified fragment (SLAF) sequencing technique using an F-2 population of 159 individuals of B. oleracea var. Alboglabra cultivars 'Dasun' and 'LBO7M' with varying flowering times. The genetic map contained 4,296 loci, 8,500 SNPs spanning 1,075.66 cM over 9 chromosomes. The linkage analysis fine mapped the loci controlling BT and FT within 0.315 and 0.314 cM, respectively. Quantitative trait loci (QTLs) analysis showed a locus on Chr 2 governing bolting time (BT) and flowering time (FT) traits, explaining 41.14 and 48.08% of phenotypic variation (PVE), respectively along with one minor QTL of BT explaining 15% of PVE on chromosome 7. The QTLs were aligned with the physical map positions of a B. oleracea reference genome. The candidate genes were preliminary annotated based on homology, located within the QTLs regions for the BT and FT traits. Regarding BT trait, two candidate genes were identified as Bo2g024555 and Bo2g009900, which control shoot apical and floral meristems, respectively. But, the gene Bo2g048220, which controls gibberellic acid production, was selected as being putatively responsible for the FT trait. In conclusion, the QTLs found in this study provide information for marker-assisted selection and could contribute to develop early maturing Chinese kale varieties. (C) 2020 Friends Science Publishers
- 相关文献
作者其他论文 更多>>
-
A Physiological and Molecular Docking Insight on Quercetin Mediated Salinity Stress Tolerance in Chinese Flowering Cabbage and Increase in Glucosinolate Contents
作者：Akram, Waheed;Khan, Imran;Guo, Juxian;Li, Guihua;Akram, Waheed;Rehman, Areeba;Munir, Bareera
关键词：salinity; Chinese flowering cabbage; Brassica rapa; quercetin dihydrate; antioxidants; foliar application
-
A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt
作者：Gong, Chao;Li, Zhiliang;Sun, Baojuan;Luo, Wenlong;Luo, Shanwei;Chen, Shuting;Mai, Peiting;Li, Zhenxing;Li, Tao;Wang, Zhenshuo;Li, Ye;Wang, Yikui
关键词：
-
Comparative Effect of Seed Coating and Biopriming of Bacillus aryabhattai Z-48 on Seedling Growth, Growth Promotion, and Suppression of Fusarium Wilt Disease of Tomato Plants
作者：Akram, Waheed;Waqar, Sara;Hanif, Sana;Anjum, Tehmina;Aftab, Zill-e-Huma;Hassan, Ali;Umer, Muhammad;Li, Guihua;Ali, Basharat;Rizwana, Humaira;Rehman, Areeba;Munir, Bareera
关键词：tomato; Fusarium wilt; plant growth-promoting bacteria; Bacillus; seed coating; biopriming
-
Integrated metabolome and transcriptome analyses reveal the role of BoGSTF12 in anthocyanin accumulation in Chinese kale (Brassica oleracea var. alboglabra)
作者：Tang, Kang;Jiang, Shizheng;Yang, Xian;Tang, Kang;Karamat, Umer;Li, Guihua;Guo, Juxian;Jiang, Shizheng;Fu, Mei
关键词：Metabolome; RNA-seq; Anthocyanins; qRT-PCR; Brassica oleracea
-
TiO2 nanoparticles: Green synthesis and their role in lessening the damage of Colletotrichum graminicola in sorghum
作者：Nabi, Ghulam;Anjum, Tehmina;Aftab, Zill-e-Huma;Akram, Waheed;Rizwana, Humaira;Akram, Waheed
关键词：Colletotrichum graminicola; green synthesis; nanoparticles; sorghum; titanium dioxide
-
Comparative Metabolome and Transcriptome Analyses Reveal the Regulatory Mechanism of Purple Leafstalk Production in Taro (Colocasia esculenta L. Schott)
作者：Jiang, Shizheng;Guo, Juxian;Khan, Imran;Tang, Kang;Li, Guihua;Fu, Mei;Jiang, Shizheng;Tang, Kang;Yang, Xian;Jahan, Mohammad Shah
关键词：taro leafstalk; metabolome; transcriptome; anthocyanins
-
Nano-Integrated Plant Tissue Culture to Increase the Rate of Callus Induction, Growth, and Curcuminoid Production in Curcuma longa
作者：Iqbal, Muhammad;Aftab, Zill-e-Huma;Anjum, Tehmina;Akram, Waheed;Rizwana, Humaira;Akram, Waheed;Li, Guihua;Aftab, Arusa;Sajid, Zahoor Ahmad
关键词：nanotechnology; turmeric; curcuminoids; Fe3O nanoparticles; antimicrobial
