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Colloidal gold-based immunochromatographic strip assay for the rapid detection of diminazene in milk

文献类型： 外文期刊

作者： Chen, Linlin 1 ; Sun, Yaning 2 ; Hu, Xiaofei 2 ; Xing, Yunrui 2 ; Kwee, Sharon 3 ; Na, Guanqiong 1 ; Zhang, Gaiping 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou, Peoples R China

3.Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX USA

关键词： Diminazene; monoclonal antibody; immunochromatographic strip; milk

期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT （ 影响因子：3.057； 五年影响因子：2.96 ）

ISSN： 1944-0049

年卷期： 2020 年 37 卷 10 期

页码：

收录情况： SCI

摘要： Diminazene (DIM) is widely used to treat trypanosomiasis in livestock, but its residues in animal products can cause food safety problems. Thus, a rapid colloidal gold-based immunochromatographic strip assay was established to analyse DIM residue in milk samples. A highly sensitive and specific monoclonal antibody (mAb) against DIM was produced with a new synthetic immunogen by the active ester method. The titre of the prepared antibody was up to 1:1.0 x 10(6), the affinity constant was 2.2 x 10(8) L mol(-1)and the half-maximal inhibitory concentration (IC50) was 0.4 ng mL(-1)based on enzyme-linked immunosorbent assay (ELISA). Then, the colloidal gold-labelled mAb probe was successfully prepared and used for preparing the immunochromatographic strip. The strip showed high sensitivity and specificity, the IC(50)for DIM was 5.2 ng mL(-1), the limit of detection was 1.2 ng mL(-1)and the linear range of detection was 1.8-15.4 ng mL(-1). The average recoveries ranged from 89.5% to 91.7%, and coefficients of variation were 6.6-7.2%. The immunochromatographic strip assay can be employed in the rapid detection of DIM residue in milk samples.

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