A double staining method using calcofluor white and acridine orange to differentiate life stages of Enterocytozoon hepatopenaei (EHP) on hepatopancreatic sections
文献类型： 外文期刊
作者： Gao, Wen 1 ; Guo, Xiao-Meng 1 ; Wang, Hai-Liang 1 ; Zhao, Ruo-Heng 1 ; Xie, Guo-Si 1 ; Li, Chen 1 ; Huang, Jie 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs,Pilot Natl Lab Marine, Yellow Sea Fisheries Res Inst,Lab Marine Fisherie, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Shanghai 201306, Peoples R China
3.Qingdao Agr Univ, Qingdao 266071, Peoples R China
关键词： Enterocytozoon hepatopenaei; Calcofluor white stain; Acridine orange; Sporophyte; Plasmodium
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2020 年 528 卷
页码：
收录情况： SCI
摘要： A double fluorescent staining method was used to differentiate life stages of Enterocytozoon hepatopenaei (EHP) with a chitin-binding dye, calcofluor white (CFW) and a nucleic acid dye, acridine orange (AO). The unreleased spores showed a significantly blue fluorescent chitin wall with weak orange-red RNA staining, while the plasmodia appeared grape cluster-like orange-red nucleic acid-containing particles without or with very weak blue fluorescence and sporophytes showed grape cluster-like orange-red particles with varying degree of blue fluorescence. The analysis of the red, blue and green intensity of different targets revealed that the ratio of relative concentration of CFW and AO (RRC/A) of plasmodia (0.36 +/- 0.15) (n = 60), sporophytes (1.25 +/- 0.41) (n = 157), and unreleased spores (3.06 +/- 1.09) (n = 21) had extremely significant differences (P < .01), which indicated an incremental relative amount of chitin to RNA in the three stages. The quantitative analysis proposed in this study indicated that double staining with CFW and AO can provide a method for differentiating various life stages of EHP in the infected hepatopancreatic cells.
- 相关文献
作者其他论文 更多>>
-
The 2023 National Proficiency Test for molecular detection of shrimp infectious myonecrosis virus (IMNV) in China
作者：Li, Chen;Yang, Bing;Wang, Meifeng;Wan, Xiaoyuan;Li, Wenqiang;Song, Xiaoling;Liu, Li;Zhang, Qingli;Li, Chen;Yang, Bing;Wan, Xiaoyuan;Zhang, Qingli;Yu, Weizhong;Li, Qing;Wang, Meifeng;Li, Wenqiang
关键词：Proficiency test (PT); Infectious myonecrosis virus (IMNV); Z-score
-
Flow Velocity Modulates Growth, Oxidative Stress, and Transcriptomic Responses in Spotted Sea Bass (Lateolabrax maculatus)
作者：Li, Shuo;Wang, Qian;Liu, Baoliang;Shao, Changwei;Li, Shuo;Liu, Yuyan;Wang, Qian;Zhu, Zhiwen;Li, Weijing;Li, Chen;Fei, Fan;Liu, Baoliang;Shao, Changwei;Liu, Yuyan;Wang, Qian;Shao, Changwei
关键词：Spotted sea bass; Flow velocity; Oxidative stress; Lipid metabolism; Transcriptomics
-
Near complete genome assembly of Yadong trout (Salmo trutta)
作者：Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Li, Chen;Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Shao, Changwei
关键词：
-
Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA
作者：Lian, Xin-Yu;Wang, Xiu-Hua;Li, Chen;Zhang, Qing-Li;Lu, Ruo-Xuan;Gou, Zi-Yue;Xu, Hua;Wang, Mei-Feng;Yang, Bing;Wang, Xiu-Hua;Zhang, Qing-Li;Yang, Bing;Lian, Xin-Yu;Wang, Xiu-Hua;Li, Chen;Zhang, Qing-Li;Lu, Ruo-Xuan;Gou, Zi-Yue;Xu, Hua;Wang, Mei-Feng;Yang, Bing;Jia, Peng
关键词：IHHNV; Flinders technology associates cards; Real-time PCR
-
High-quality chromosome-level genome assembly of the whitespotted conger (Conger myriaster)
作者：Han, Shenglei;Han, Shenglei;Li, Chen;Li, Shuo;Chen, Yan;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shi, Bao;Shao, Changwei;Han, Shenglei;Li, Chen;Li, Shuo;Chen, Yan;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shi, Bao;Shao, Changwei;Wang, Chenggang;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-Yan;Wang, Qian;Cao, Xuwen;Shao, Changwei
关键词：
-
Evolutionary and functional insights into Fibrinogen-related protein (FREP) dynamics in sea cucumbers
作者：Li, Shuo;Chen, Kaiyu;Li, Chenghua;Li, Shuo;Li, Chen;Han, Shenglei;Li, Weijing;Jiang, Shuhong;Liao, Duan;Shao, Changwei;Li, Shuo;Li, Chen;Han, Shenglei;Li, Weijing;Jiang, Shuhong;Liao, Duan;Shao, Changwei
关键词：Fibrinogen-related proteins; Sea cucumber; Gene duplication and diversification; Immune adaptation; Chromosomal rearrangements
-
Penaeus chinensis nearing a critical threshold of susceptibility to Decapod iridescent virus 1 (DIV1)
作者：Guo, Xiao-Meng;Wang, Guo-Hao;Xing, Jing-Yi;Qiu, Liang;Huang, Jie;Qiu, Liang;Huang, Jie;Yao, Jia-Yun;Guo, Xiao-Meng;Wang, Guo-Hao;Huang, Jie;Chen, Xiao-Ling;Xing, Jing-Yi
关键词：Decapod iridescent virus 1; Susceptible species; Penaeus chinensis; Non-invasive challenge mimicking nature; transmission pathway
