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A rapid method of sex-specific marker discovery based on NGS and determination of the XX/XY sex-determination system in Channa maculata

文献类型： 外文期刊

作者： Yang, Cheng 1 ; Huang, Rong 1 ; Ou, Mi 3 ; Gui, Bin 1 ; Zhao, Jian 3 ; He, Libo 1 ; Li, Yongming 1 ; Liao, Lanjie 1 ; Chen, 1 ;

作者机构： 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr, Guangzhou 510380, Peoples R China

4.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China

关键词： Sex-specific marker; Next-generation sequencing (NGS); Sex-determination mechanism; Unisexual breeding

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 528 卷

页码：

收录情况： SCI

摘要： The discovery of sex-specific markers is of great significance for the study of unisexual breeding and sex-determination mechanisms of fish. The all-male hybrid snakehead is a freshwater aquaculture species with high economic value. The breeding of all-male hybrid snakeheads depends on the discovery of sex-specific markers of Taiwan snakeheads. In this study, 24 females and 24 males of Taiwan snakeheads were sequenced by NGS, and then a set of biological information analysis process was developed to speculate that Taiwan snakeheads were XX / XY sex-determining system, and 1250 male-related contigs were assembled; In combination with indels and SNP sites analysis, Chelex 100 DNA extraction and an HRM scan, two molecular markers (CM286 and CM1053) were selected from 29 male-related contigs, which could clearly distinguish XX females, XY males, and YY supermales; Then, it was confirmed by Sanger sequencing that Taiwan snakeheads had a male heterogamete type, and the ratio of females to males in three full-sib families was all close to 1:1 (P >.05). Therefore, Taiwan snakeheads were determined to be the XX / XY sex-determining system. The results showed that the effective sex-specific markers, combined with the Chelex 100 DNA extraction and HRM scan technology, could realize high-throughput and rapid detection of the three sexes (XX, XY, and YY) of Taiwan snakeheads, and could be directly applied to the breeding of the all-male hybrid snakehead. This process of bioinformatic analysis for sexspecific marker discovery is simple and efficient, and can also be applied to the study of other fish sex-specific marker discovery and sex-determination mechanisms.

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