Cloning and expression of a novel alpha-galactosidase fromLactobacillus amylolyticusL6 with hydrolytic and transgalactosyl properties
文献类型： 外文期刊
作者： Fei, Yongtao 1 ; Jiao, WenJuan 2 ; Wang, Ying 1 ; Liang, Jinglong 1 ; Liu, Gongliang 1 ; Li, Li 3 ;
作者机构： 1.Zhongkai Univ Agr & Engn, Coll Light Ind & Food Sci, Guangzhou, Peoples R China
2.Guangdong Acad Agr Sci, Sericultural Agri Food Res Inst, Guangzhou, Peoples R China
3.South China Univ Technol, Sch Food Sci & Engn, Guangzhou, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2020 年 15 卷 7 期
页码：
收录情况： SCI
摘要： Lactobacillus amylolyticusL6, a gram-positive amylolytic bacterium isolated from naturally fermented tofu whey (NFTW), was able to hydrolyze raffinose and stachyose for the production of alpha-galactosidase. The cell-free extract ofL.amylolyticusL6 was found to exhibit glycosyltransferase activity to synthesize alpha-galacto-oligosaccharides (GOS) with melibiose as substrate. The coding genes of alpha-galactosidase were identified in the genome ofL.amylolyticusL6. The alpha-galactosidase (AglB) was placed into GH36 family by amino acid sequence alignments with other alpha-galactosidases from lactobacilli. The optimal reaction conditions of pH and temperature for AglB were pH 6.0 and 37 degrees C, respectively. Besides, potassium ion was found to improve the activity of AglB while divalent mercury ion, copper ion and zinc ion displayed different degrees of inhibition effect. Under the optimum reaction condition, AglB could catalyze the synthesis of GOS with degree of polymerization (DP) >= 5 by using 300 mM melibiose concentration as substrate. The maximum yield of GOS with (DP) >= 3 could reach 31.56% (w/w). Transgalactosyl properties made AglB a potential candidate for application in the production of GOS.
- 相关文献
作者其他论文 更多>>
-
Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)
作者：Wang, Min;Liu, Wenrui;Peng, Qingwu;Shi, Shaoqi;Jiang, Biao;Lin, Yu'e;Yang, Songguang;Wang, Min;Liu, Wenrui;Shi, Shaoqi;Cao, Liqin;Jiang, Biao;Zhao, Tianyue;Cui, Xiaojuan;Yang, Songguang;Wang, Ying
关键词：bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress
-
Development, evaluation and application of a polymerase spiral reaction (PSR) based assay on accurate detection of Vibrio parahaemolyticus viable cells and virulence factors from rice product
作者：Liu, Junyan;Liu, Gongliang;Qu, Chunyun;Liu, Junyan;Liu, Gongliang;Qu, Chunyun;Huang, Tengyi;Xu, Zhenbo;Xiang, Zhufang;Soteyome, Thanapop;Luo, Yuting;Li, Xuejie;Ma, Qin;Yuan, Lei;Hong, Wei;Li, Xuejie;Ye, Yanrui
关键词：Vibrio parahaemolyticus; Food safety; Polymerase spiral reaction (PSR); Rapid detection; VBNC cells
-
Colloidal fraction on pomelo peel-derived biochar plays a dual role as electron shuttle and adsorbent in controlling arsenic transformation in anoxic paddy soil
作者：Zhong, Ruilin;Pan, Dandan;Huang, Guoyong;Yang, Guang;Wang, Xiaonan;Niu, Rumiao;Ding, Ziman;Chi, Wenting;Li, Xiaomin;Pan, Dandan;Huang, Guoyong;Chi, Wenting;Wang, Ying;Cai, Xixi
关键词：Biochar colloids; As(V) reduction; Electron transfer capability; As(III) immobilization; Adsorption
-
Fabrication, characterization, and dihydromyricetin-loaded bioavailability of Pickering emulsions stabilized by Ampelopsis grossedentata polysaccharide -fish collagen peptide composite nanoparticles
作者：Pan, Haihui;Wang, Feilin;Sheng, Yuanhao;Kong, Fansheng;Pan, Haihui;Jiao, Wenjuan;Zhang, Yousheng
关键词：Fish collagen peptide; Ampelopsis grossedentata polysaccharide; Nanocomplexes; Pickering emulsion; Bioavailability
-
Formulation and stabilization of high internal phase emulsions via mechanical cellulose nanofibrils/ethyl lauroyl arginate complexes
作者：Huang, Luyao;Xu, Chuan;Gao, Wenhua;Guo, Shasha;Li, Jun;Rojas, Orlando J.;Jiao, Wenjuan;Jiao, Wenjuan
关键词：Cellulose nanofibrils; Ethyl lauroyl arginate; High internal phase emulsions; 3D printing
-
Differential selection of yield and quality traits has shaped genomic signatures of cowpea domestication and improvement
作者：Wu, Xinyi;Li, Mao;Dong, Junyang;Wang, Baogen;Wu, Jian;Wu, Xiaohua;Wang, Ying;Wang, Jian;Lu, Zhongfu;Sun, Yuyan;Dong, Wenqi;Li, Guojing;Hu, Zhongyuan;Liao, Nanqiao;Zhang, Mingfang;Zhang, Yan;Yang, Yi;Li, Mao;Dong, Junyang;Wang, Baogen;Wu, Jian;Wu, Xiaohua;Wang, Ying;Wang, Jian;Lu, Zhongfu;Sun, Yuyan;Dong, Wenqi;Li, Guojing;Zhang, Mingfang;Zhang, Mingfang
关键词：
-
Genome-Wide Identification of SnRK1 Catalytic alpha Subunit and FLZ Proteins in Glycyrrhiza inflata Bat. Highlights Their Potential Roles in Licorice Growth and Abiotic Stress Responses
作者：Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Yang, Chao;Shi, Guangyu;Li, Yuping;Luo, Ming;Wang, Ying;Li, Yongqing;Wang, Hongxia;Wang, Jihua;Yuan, Ling;Wang, Ying
关键词：SnRK1; FLZ; Glycyrrhiza inflata; stress response; growth
