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Molecular mechanisms underpinning the multiallelic inheritance ofMS5inBrassica napus

文献类型： 外文期刊

作者： Xin, Qiang 1 ; Wang, Xiang 1 ; Gao, Yupeng 1 ; Xu, Dongdong 1 ; Xie, Zhaoqi 1 ; Dong, Faming 1 ; Wan, Lili 2 ; Yang, Liyo 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

2.Wuhan Acad Agr Sci, Inst Crop, Wuhan 430065, Hubei, Peoples R China

3.Shanghai Acad Agr Sci, Shanghai 201403, Peoples R China

关键词： male sterility; triallelic inheritance; dominant-negative effect; Brassica napus; CRISPR; Cas9-directed mutation

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2020 年 103 卷 5 期

页码：

收录情况： SCI

摘要： TheBrassica-specific geneMS5mediates early meiotic progression, and its allelic variants contribute to a valuable genic male sterility three-line hybrid production system in rapeseed (Brassica napusL.). However, the underlying mechanisms of its triallelic inheritance are poorly understood. Herein, we show that the restorer alleleMS5(a)and the maintainer alleleMS5(C)are both necessary for male fertility inB. napus. The functional divergence ofMS5(a)andMS5(C)is strongly related to sequence variations in their coding regions and less strongly to their promoter regions. The male-sterile alleleMS5(b)encodes a chimeric protein containing only the complete MS5 coiled-coil (CC) domain, having lost the MS5 superfamily domain. Both MS5(a)and MS5(C)can form homodimers in the nucleus via the CC domain. MS5(b)can interact competitively with MS5(a)or MS5(C)to form non-functional heterodimers. Owing to the close transcript levels ofMS5(b)andMS5(C)inMS5(b)MS5(C) these heterodimers induced a dominant-negative effect ofMS5(b)onMS5(C)resulting in a male-sterile phenotype. The extremely high transcript abundance ofMS5(a)maintains sufficient MS5(a)homodimers inMS5(a)MS5(b), causing the recovery of male sterility. These findings provide substantial genetic and molecular evidence to improve our understanding of the mechanisms underlying the multiallelic inheritance ofMS5, and enable the construction of a solid foundation for improved use of theMS5-controlled GMS system inBrassicaspecies.

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