Ecdysone Receptor (EcR) and Ultraspiracle Protein (USP) Genes FromConopomorpha sinensisBradley Eggs: Identification and Expression in Response to Insecticides
文献类型： 外文期刊
作者： Yao, Qiong 1 ; Xu, Shu 1 ; Dong, Yizhi 1 ; Quan, Linfa 1 ; Chen, Bingxu 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Guangdong Prov Key Lab New High Technol Plant Pro, Plant Protect Res Inst, Guangzhou, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China
关键词： litchi fruit borer; EcR; USP; ovicide; biomarker
期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）
ISSN： 1664-042X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Conopomorpha sinensisBradley (Lepidoptera: Gracilariidae) is the dominant insect pest of litchi (chinensisSonn.) and longan (Euphoria longanLour.) fruit trees. Management of this pest species is a challenging task due to its cryptic borer behavior. ControllingC. sinensisat the egg stage is the best alternative strategy to chemical control ofC. sinensisadults. However, thorough studies regarding the indirect and sublethal effects of chemicals on the different developmental stages ofC. sinensisare insufficient. In this study, the effect of some insecticides was evaluated onC. sinensiseggs. The ovicidal activity of chlorbenzuron, abamectin, chlorantraniliprole, and lambda-cyhalothrin was confirmed by morphological observation of the defects inC. sinensiseggs. Moreover, we characterized four essential ecdysone receptor proteins in insects [i.e., two isoform ecdysone receptors (EcR: CsEcRA. CsEcRB) and two isoform ultraspiracle proteins (USP:CsUSP1,CsUSP2)] fromC. sinensiseggs. TheCsEcRA,CsEcRB,CsUSP1, andCsUSP2genes consisted of 1521-, 1614-, 1410-, and 1236-bp open reading frames which encoded proteins of 506, 527, 469, and 413 amino acid residues, respectively. Furthermore, the embryonic differential responses ofCsEcRs,CsUSPs, and vitellogenin receptor (VgR: CsVgR) to insecticides were evaluated by qRT-PCR. Among the five tested genes,CsVgRandCsUSP1were the most sensitive to all the tested insecticides, with fold change of the expression diminished by 4.27-8.70 times compared with untreated control insects. The data suggests that these insecticidal compounds regulate the expression of these specific proteins, which might eventually lead to reduced viability ofC. sinensiseggs. We present here the first data providing molecular elucidation of ecdysone receptor genes and their differential responses to insecticides inC. sinensiseggs. Together with our previous report of insecticide sublethal effects on two reproduction-related genes inC. sinensisadults,CsVgRandCsUSP1seem to be appropriate molecular parameters for the evaluation of insecticide impact onC. sinensis. This study exemplifies the potential utility of transcriptional measurement of nuclear receptors as the molecular biomarkers for ecotoxicological evaluations of ovicidal impact of insecticides.
- 相关文献
作者其他论文 更多>>
-
Identification and characterization of circadian clock genes in the head transcriptome of Conopomorpha sinensis Bradley
作者：Quan, Lin-fa;Chi, Yan-yan;Dong, Yi-Zhi;Xu, Shu;Chen, Bing-xu;Li, Wen-jing
关键词：Circadian clock gene; Conopomorpha sinensis; Head transcriptome; Expression profile
-
Knowledge graph and deep learning based pest detection and identification system for fruit quality
作者：Zhu, DingJu;Xie, LianZi;Tan, JianBin;Zheng, Yongzhi;Hu, Qi;Yi, Shuai;Zhu, DingJu;Deng, RenFeng;Zhu, DingJu;Chen, BingXu;Mustafa, Rashed;Chen, Wanshan;Yung, KaiLeung;Andrew, W. H. Ip
关键词：Pests detection and identification; Knowledge graph; Raspberry PI; Image classification
-
IPS (In-Plant System) Delivery of Double-Stranded Vitellogenin and Vitellogenin receptor via Hydroponics for Pest Control in Diaphorina citri Kuwayama (Hemiptera: Psyllidae)
作者：Li, Hailin;Xu, Shu;Li, Hailin;Mo, Junlan;Wang, Xiaoyun;Pan, Biqiong;Li, Shuangrong;Zheng, Xialin;Lu, Wen
关键词：Diaphorina citri; RNAi; vitellogenin; egg formation; integrated pest management
-
Evidence for the Participation of Chemosensory Proteins in Response to Insecticide Challenge in Conopomorpha sinensis
作者：Yao, Qiong;Chen, Bingxu;Liang, Zhantu
关键词：chemosensory proteins; A-cyhalothrin; molecular docking
-
Sublethal effect of chlorpyrifos on predatory behavior and physiology of Eocanthecona furcellata (Hemiptera: Pentatomidae)
作者：Xu, Shu;Zeng, Dongqiang;Xu, Shu;Yao, Qiong;Quan, Linfa;Dong, Yizhi;Chen, Bingxu
关键词：chlorpyrifos; sublethal exposure; functional response; metabolic enzyme; gene expression
-
Functional characterization of chemosensory proteins in response to artificial light treatment in Thalassodes immissaria
作者：Yao, Qiong;Quan, Linfa;Chen, Bingxu;Liang, Zhantu
关键词：Thalassodes immissaria; chemosensory protein; sequence analysis; molecular docking; artificial light at night; stress response
-
Predatory stink bug, Eocanthecona furcellata (Wolff) responses to oral exposure route of lambda-cyhalothrin via sex-specific modulation manner
作者：Yao, Qiong;Quan, Linfa;Wang, Siwei;Chen, Bingxu;Lu, Kai;Xing, Dongxu
关键词：Pesticides; Toxin uptake and accumulation; Reproductive potential; Predators; Enzymatic properties
