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IPS (In-Plant System) Delivery of Double-Stranded Vitellogenin and Vitellogenin receptor via Hydroponics for Pest Control in Diaphorina citri Kuwayama (Hemiptera: Psyllidae)

文献类型： 外文期刊

作者： Li, Hailin 1 ; Mo, Junlan 2 ; Wang, Xiaoyun 2 ; Pan, Biqiong 2 ; Xu, Shu 1 ; Li, Shuangrong 2 ; Zheng, Xialin 2 ; Lu, Wen 2 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab Green Prevent & Control Fruits, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

2.Guangxi Univ, Coll Agr, Guangxi Key Lab Agr Environm & Agr Prod Safety, Nanning 530004, Peoples R China

关键词： Diaphorina citri; RNAi; vitellogenin; egg formation; integrated pest management

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 11 期

页码：

收录情况： SCI

摘要： Diaphorina citri, a vector of citrus huanglongbing (HLB) disease, frequently leads to HLB outbreaks and reduces Rutaceae crop production. Recent studies have investigated the effects of RNA interference (RNAi) targeting the Vitellogenin (Vg4) and Vitellogenin receptor (VgR) genes, which are involved in egg formation in this pest, providing a theoretical foundation for developing new strategies to manage D. citri populations. This study presents RNAi methods for Vg4 and VgR gene expression interference and reveals that dsVgR is more effective than dsVg4 against D. citri. We demonstrated that dsVg4 and dsVgR persisted for 3-6 days in Murraya odorifera shoots when delivered via the in-plant system (IPS) and effectively interfered with Vg4 and VgR gene expression. Following Vg4 and VgR gene expression interference, egg length and width in the interference group were significantly smaller than those in the negative control group during the 10-30-day development stages. Additionally, the proportion of mature ovarian eggs in the interference group was significantly lower than that in the negative control group at the 10, 15, 20, 25, and 30-day developmental stages. DsVgR notably suppresses oviposition in D. citri, with fecundity decreasing by 60-70%. These results provide a theoretical basis for controlling D. citri using RNAi to mitigate the spread of HLB disease.

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