SSEL, a selective enrichment broth for simultaneous growth ofSalmonella enterica,Staphylococcus aureus,Escherichia coliO157:H7, andListeria monocytogenes
文献类型： 外文期刊
作者： Qu, Yang 1 ; Bai, Yalong 1 ; Liu, Yanhong 2 ; Zhou, Changyan 1 ; Zhou, Xiujuan 3 ; Zhang, Donglai 1 ; Shi, Chunlei 3 ; Su 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China
2.ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA
3.Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Sch Agr & Biol, State Key Lab Microbial Metab, Shanghai, Peoples R China
期刊名称：JOURNAL OF FOOD SAFETY （ 影响因子：1.953； 五年影响因子：1.946 ）
ISSN： 0149-6085
年卷期： 2020 年 40 卷 5 期
页码：
收录情况： SCI
摘要： Salmonella enterica,Staphylococcus aureus,Escherichia coliO157: H7, andListeria monocytogenesmay contaminate similar types of food and cause foodborne disease. The objective of this study was to develop a selective enrichment broth for simultaneous enrichment ofSalmonella enterica,Staphylococcus aureus,Escherichia coliO157: H7, andListeria monocytogenes(SSEL) using nalidixic acid, acriflavine, lithium chloride, and sodium cholate as selective agents. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37 degrees C with 10-100 CFU/mL of inoculation concentration, but also could successfully support the simultaneous enrichment of target pathogens with similar growth rates and inhibit the growth of most nontarget bacteria effectively. The enrichment effect of SSEL was confirmed by artificial contamination test coupled with multiplex PCR. In summary, SSEL has been shown to be a promising multiplex selective enrichment broth for the detection of the four pathogens on a single-assay platform.
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