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MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar

文献类型： 外文期刊

作者： Wang, Yamei 1 ; Liu, Wenwen 1 ; Wang, Xinwei 3 ; Yang, Ruijuan 1 ; Wu, Zhenying 1 ; Wang, Han 1 ; Wang, Lei 4 ; Hu, Zhubi 1 ;

作者机构： 1.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 10083, Peoples R China

4.Henan Univ, Collaborat Innovat Ctr Crop Stress Biol, Henan Prov & Inst Plant Stress Biol, Kaifeng 475001, Peoples R China

5.Heilongjiang Acad Agr Sci, Grass & Sci Inst, Harbin, Heilongjiang, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：6.793； 五年影响因子：6.589 ）

ISSN： 2662-6810

年卷期： 2020 年 7 卷 1 期

页码：

收录情况： SCI

摘要： Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses. Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries. However, little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar. Here, we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins. Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels. Furthermore, integrated microRNAomic and transcriptomic analysis suggested that two microRNAs, miR160h, and miR858, have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors, respectively. Additionally, the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels, suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar. Last, metabolomics analysis revealed that the levels of anthocyanins, flavones, and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type, whereas the total lignin content was reduced in the transgenic plants. Taken together, our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors, including microRNAs, transcription factors, and the levels of structural genes, in poplar. This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.

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