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A novel CCCH-type zinc finger protein SAW1 activatesOsGA20ox3to regulate gibberellin homeostasis and anther development in rice

文献类型： 外文期刊

作者： Wang, Bin 1 ; Fang, Ruiqiu 1 ; Chen, Faming 1 ; Han, Jingluan 1 ; Liu, Yao-Guang 1 ; Chen, Letian 1 ; Zhu, Qinlong 1 ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Peoples R China

2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

3.South China Agr Univ, Guangdong Prov Key Lab Prot Funct & Regulat Agr O, Guangzhou 510642, Peoples R China

4.South China Agr Univ, Coll Life Sci, Guangzhou 510642, Peoples R China

5.Zhejiang Acad Agr Sci, Dongyang Inst Maize Res, Dongyang 322100, Peoples R China

关键词： anther development; CCCH proteins; CRISPR; Cas9; gibberellin; rice

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2020 年 62 卷 10 期

页码：

收录情况： SCI

摘要： Male sterility is a prerequisite for hybrid seed production. The phytohormone gibberellin (GA) is involved in regulating male reproductive development, but the mechanism underlying GA homeostasis in anther development remains less understood. Here, we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1), for anther development in rice (Oryza sativaL.). Rice plants carrying the recessive mutant allelesaw1produces abnormal anthers with swollen anther wall and aborted pollen. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRIPSR-associated protein 9-mediated knockout ofSAW1in rice generated similar male sterile plants.SAW1encodes a novel nucleus-localizing CCCH-tandem zinc finger protein, and this protein could directly bind to the promoter region of the GA synthesis geneOsGA20ox3to induce its anther-specific expression. In thesaw1anther, the significantly decreasedOsGA20ox3expression resulted in lower bioactive GA content, which in turn caused the lower expression of the GA-inducible anther-regulator geneOsGAMYB. Thus, our results disclose the mechanism of theSAW1-GA20ox3-GAMYBpathway in controlling rice anther development, and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding.

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