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Alterations of Rice (Oryza sativaL.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus

文献类型： 外文期刊

作者： Li, Linying 1 ; He, Yuqing 2 ; Zhang, Xueying 2 ; Zhang, Hehong 3 ; Sun, Zongtao 3 ; Li, Junmin 3 ; Hong, Gaojie 2 ;

作者机构： 1.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, 198 Shiqiao Rd, Hangzhou 310021, Peoples R China

3.Ningbo Univ, Inst Plant Virol, State Key Lab Breeding Base Sustainable Control P, Ningbo 315211, Peoples R China

关键词： DNA methylation; rice; RBSDV; differentially methylated genes; gene expression

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 16 期

页码：

收录情况： SCI

摘要： Rice black-streaked dwarf virus (RBSDV) causes severe yield losses in rice (Oryza sativaL.) in China. Studies have shown that the mechanisms of DNA methylation-mediated plant defense against DNA viruses and RNA viruses are different. However, in rice its function in response to infection of RBSDV, a double-stranded RNA virus, remains unclear. In this study, high-throughput single-base resolution bisulfite sequencing (BS-Seq) was carried out to analyze the distribution pattern and characteristics of cytosine methylation in RBSDV-infected rice. Widespread differences were identified in CG and non-CG contexts between the RBSDV-infected and RBSDV-free rice. We identified a large number of differentially methylated regions (DMRs) along the genome of RBSDV-infected rice. Additionally, the transcriptome sequencing analysis obtained 1119 differentially expressed genes (DEGs). Correlation analysis of DMRs-related genes (DMGs) and DEGs filtered 102 genes with positive correlation and 71 genes with negative correlation between methylation level at promoter regions and gene expression. Key genes associated with maintaining DNA methylation in rice were analyzed by RT-qPCR and indicated thatOsDMT702might be responsible for the global increase of DNA methylation level in rice under RBSDV stress. Our results suggest important roles of rice DNA methylation in response to RBSDV and provide potential target genes for rice antiviral immunity.

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