Comparative Genomic and Secretomic Analysis Provide Insights Into Unique Agar Degradation Function of Marine BacteriumVibrio fluvialisA8 Through Horizontal Gene Transfer
文献类型： 外文期刊
作者： Li, Chunsheng 1 ; Li, Chi 1 ; Li, Laihao 1 ; Yang, Xianqing 1 ; Chen, Shengjun 1 ; Qi, Bo 1 ; Zhao, Yongqiang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Natl R&D Ctr Aquat Prod Proc, Key Lab Aqut Prod Proc,Minist Agr & Rural Affairs, Guangzhou, Peoples R China
2.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang, Peoples R China
关键词： complete genome; secretome; agarase; horizontal gene transfer; Vibrio
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Agarose-oligosaccharide production from agar degradation by agarase exhibits lots of advantages and good application prospects. In this study, a novel agar-degrading bacteriumVibriosp. A8 was isolated from a red algae in the South China Sea. The whole genome sequencing with comparative genomic and secretomic analysis were used to better understand its genetic components about agar degradation. This strain exhibited good agarase production in artificial seawater after culture optimization. The complete genome (4.88 Mb) of this strain comprised two circular chromosomes (3.19 and 1.69 Mb) containing 4,572 protein-coding genes, 108 tRNA genes and 31 rRNA genes. This strain was identified asVibrio fluvialisA8 by comparative genomic analysis based on genome phylogenetic tree and average nucleotide identity (ANI) similarity. Different from other 20 similar strains including three strains of the same species,V. fluvialisA8 possessed unique agar degradation ability with four beta-agarases (GH50) and one alpha-1,3-L-NA2 hydrolase (GH117) due to the horizontal gene transfer. Secretomic analysis showed that only beta-agarase (gene 3152) was abundantly expressed in the secretome ofV. fluvialisA8. This agarase had a good substrate specificity and wide work conditions in complex environments, suggesting its potential application for agarose-oligosaccharide production.
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