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Negligible transcriptome and metabolome alterations in RNAi insecticidal maize againstMonolepta hieroglyphica

文献类型： 外文期刊

作者： Zhang, Xiaolei 1 ; Zhang, Ruiying 1 ; Li, Liang 2 ; Yang, Yang 1 ; Ding, Yijia 1 ; Guan, Haitao 1 ; Wang, Xiaoqin 1 ; Zha 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Qual & Safety Inst Agr Prod, Harbin 150086, Peoples R China

2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

3.Beijing DaBeiNong Biotechnol Co Ltd, Beijing 100080, Peoples R China

关键词： Transcriptome; Metabolome; RNAi insecticidal maize; Monolepta hieroglyphica(motschulsky); Unintended effect

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2020 年 39 卷 11 期

页码：

收录情况： SCI

摘要： Key message RNAi-based genetically modified maize resistant to Monolepta hieroglyphica (Motschulsky) was demonstrated with negligible transcriptome and metabolome alterations compared to its unmodified equivalent. As one of the most prevalent insect pests afflicting various crops,Monolepta hieroglyphica(Motschulsky) causes severe loss of agricultural and economic productivity for many years in China. In an effort to reduce damages, in this study, an RNA interference (RNAi)-based genetically modified (GM) maize was developed. It was engineered to produceMhSnf7double-stranded RNAs (dsRNAs), which can suppress theSnf7gene expression and then leadM. hieroglyphicato death.Field trail analysis confirmed the robustly insecticidal ability of theMhSnf7GM maize to resist damages byM. hieroglyphica. RNA sequencing analysis identified that only one gene was differentially expressed in theMhSnf7GM maize compared to non-GM maize, indicating that the transcriptome inMhSnf7GM maize is principally unaffected by the introduction of theMhSnf7dsRNA expression vector. Likewise, metabolomics analysis identified that only 8 out of 5787 metabolites were significantly changed. Hence, the integration of transcriptomics and metabolomics demonstrates that there are negligible differences betweenMhSnf7GM maize and its unmodified equivalent. This study not only presents a comprehensive assessment of cellular alteration in terms of gene transcription and metabolite abundance in RNAi-based GM maize, but also could be used as a reference for evaluating the unintended effect of GM crops.

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