Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein
文献类型： 外文期刊
作者： Ge, Shikun 1 ; Xu, Long 1 ; Li, Ben 1 ; Zhong, Fagang 4 ; Liu, Xiang 1 ; Zhang, Xiaoying 1 ;
作者机构： 1.Shaanxi Univ Technol, Sch Biol Sci & Engn, Key Lab Biol Resources & Ecol Environm Qinba A, Chinese German Joint Lab Nat Prod Res, Hanzhong, Peoples R China
2.Univ Minho, Ctr Mol & Environm Biol, Dept Biol, Campus Gualtar, P-4710057 Braga, Portugal
3.Univ Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON, Canada
4.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China
关键词： IgY-Technology; infectious animal diseases; virus diseases; parvovirus; phage display system
期刊名称：VETERINARY RESEARCH （ 影响因子：3.683； 五年影响因子：4.106 ）
ISSN： 0928-4249
年卷期： 2020 年 51 卷 1 期
页码：
收录情况： SCI
摘要： Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2. The IgY single chain fragment variables (scFv) were generated by T7 phage display system and expressed inE. colisystem. The titer of the primary scFv library reached to 1.5 x 10(6)pfu/mL, and 95% of the phages contained the target fragments. The CPV-VLP and CPV-VP2 protein showed similar reaction values to the purified scFv in the ELISA test, and the results of ELISA analysis using IgY-scFv toward CPV clinical samples were consistent with commercial immunochromatographic assay (ICA) and PCR detection, the scFv did not show cross reactivity with canine distemper virus (CDV) and canine coronavirus (CCV). IgY-scFv was successfully expressed in CRFK cells, and in the virus suppression assay, 55% of CPV infections were eliminated within 24 h. Docking results demonstrated that the number of amino acids of the binding sides between scFv and VP2 were AA37 and AA40, respectively. This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.
- 相关文献
作者其他论文 更多>>
-
Adenylate Cyclase Affects the Virulence of Extraintestinal Pathogenic Escherichia coli Derived from Sheep Lungs
作者：Chai, Yingjin;Huang, Xin;Han, Mengli;Zhong, Fagang;Zhang, Xingxing;Wu, Tongzhong;Chai, Yingjin;Gu, Xiaoxiao;Li, Jie;Wang, Xiaolan;Zhou, Xia;Sun, Yingni
关键词：Adhesion and invasion; cyaA; Median lethal dose; XJ10
-
Isolation, Identification and Antibacterial Resistance Spectrum of Bacterial Pathogens of Respiratory Tract Infection in a Large-Scale Cattle Farm
作者：Guo, Yunxia;Zhou, Xia;Huang, Xin;Zhong, Fagang
关键词：Respiratory tract infections; Antimicrobial; Susceptibility; Bovine; Pneumonia
-
CircRNA-02191 regulating unsaturated fatty acid synthesis by adsorbing miR-145 to enhance CD36 expression in bovine mammary gland
作者：Chen, Zhi;Han, Mengli;Li, Guoqing;Zhang, Bin;Yang, Yang;Zhong, Fagang;Dai, Rong;Chen, Zhi;Wang, Yuhao;Wang, Kun;Zhang, Zhenbin;Yang, Zhangping;Wang, Mengzhi;Loor, Juan J.;Loor, Juan J.
关键词：CircRNA-02191; MiR-145; CD36; Bovine mammary gland; Unsaturated fatty acid
-
Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China
作者：Gu, Xiaoxiao;Ma, Xue;Wu, Qin;Tao, Qiaoxiaoci;Chai, Yingjin;Zhou, Xia;Li, Jie;Cao, Yiheng;Zhang, Liyuan;Han, Mengli;Huang, Xin;Wu, Tongzhong;Zhang, Xingxing;Zhong, Fagang
关键词：antibiotic resistance; cattle; Escherichia coli infections; multiplex polymerase chain reaction; phylogeny; virulence factors; sheep
-
Seasonal Differences in Fecal Microbial Community Structure and Metabolism of House-Feeding Chinese Merino Fine-Wool Sheep
作者：Zhang, Xingxing;Han, Mengli;Guo, Yanhua;Huang, Xin;Wu, Tongzhong;Wang, Limin;Zhang, Yiyuan;Tang, Hong;Zhang, Qian;Zhou, Ping;Zhong, Fagang;Zhang, Xingxing;Han, Mengli;Guo, Yanhua;Huang, Xin;Wu, Tongzhong;Wang, Limin;Zhang, Yiyuan;Tang, Hong;Zhang, Qian;Zhou, Ping;Zhong, Fagang;Li, Chuang;Wang, Mengzhi;Shahzad, Khuram
关键词：microbial community structure; season; Chinese merino fine-wool sheep; 16S rRNA gene; metabolic pathway; blood routine
-
Isolation, Identification, and Genetic Phylogenetic Analysis of Two Different Genotypes of Bovine Parainfluenza 3 Virus in China
作者：Wang, Xu;Meng, Fanyan;Cao, Yiheng;Wang, Zijie;Zhang, Qian;Zhang, Xingxing;Han, Mengli;Wu, Tongzhong;Zhong, Fagang;Huang, Xin;Wang, Xu;Hu, Jianjun;Meng, Fanyan;Wang, Xu;Zhang, Qianyi
关键词：bovine parainfluenza virus type 3; genetic phylogenetic analysis; virus identification; indirect immunofluorescence; virus isolation; next-generation sequencing
-
Generation and evaluation of IgY-scFv based mimetics against canine parvovirus
作者：Ge, Shikun;Liu, Xiang;Zhang, Xiaoying;Ge, Shikun;Zhang, Xiaoying;Zhang, Xiaoying;Zhang, Xingxing;Zhong, Fagang;Zhang, Xiaoying
关键词：Antibody mimetics; IgY-peptide; Canine parvovirus (CPV); IgY-scFv; IgY technology
