Extraction Optimization, Purification and Immunostimulatory Activity in vitro of Polyphenols from Apple (Malus domestica) Peel
文献类型： 外文期刊
作者： Riaz, Asad 1 ; Yousaf, Khurram 2 ; Abdin, Mohamed 1 ; Jabbar, Saqib 3 ; Abid, Muhammad 4 ; Hashim, Malik Muhammad 5 ;
作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Coll Engn, Nanjing 210031, Jiangsu, Peoples R China
3.Natl Agr Res Ctr, Food Sci & Prod Dev Inst, Islamabad, Pakistan
4.Pir Mehr Ali Shah Arid Agr Univ, Dept Food Technol, Rawalpindi, Pakistan
5.Goma Univ, Dept Food Sci & Technol, Dera Ismail Khan, Pakistan
6.Jiangsu Acad Agr Sci, Inst Ago Prod & Proc, Nanjing, Jiangsu, Peoples R China
关键词： Apple peel; extraction; immunostimulating activity; polyphenols; purification; RAW264.7
期刊名称：SAINS MALAYSIANA （ 影响因子：1.009； 五年影响因子：0.95 ）
ISSN： 0126-6039
年卷期： 2020 年 49 卷 7 期
页码：
收录情况： SCI
摘要： Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction o f polyphenols were optimized by the response surface methodology The optimized conditions determined as 32 min of extraction time, 29 degrees C of extraction temperature and ethanol concentration of 56% with a yield of 35.08 +/- 0.26 mg gallic acid equivalent/g dry weight. Six resins were used for the purification and D101 resin showed the highest ratio of desorption for polyphenols and further applied for dynamic adsorption/desorption test. Catechin, procyandin B, querecitin-3-galactoside and querecitin xyloside were identified in purified polyphenols by HPLC-MS/MS. Finally the immunostimulatory activity in vitro of purified polyphenols on RAW264.7 cell lines was evaluated The apple peel polyphenols exhibited a dose-dependent effect (p < 0.05) on the strongest propagation of RAW264.7 cells. The results demonstrate that apple peel is a valuable source of polyphenols and could be used as a natural immunostimulating agent for application in functional foods.
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