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Molecular cloning, sequence characteristics, and tissue expression analysis of glucagon receptor gene in Bama minipig

文献类型： 外文期刊

作者： An, Cuiping 1 ; Zhang, Kaiyi 1 ; Zhu, Wenjuan 1 ; Bi, Yanzhen 2 ; Wu, Tianwen 1 ; Tao, Cong 1 ; Wang, Yanfang 1 ; Yang, S 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Minist Agr, Key Lab Anim Nutr,Key Lab Anim Genet Breeding & R, Beijing 100193, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Wuhan 430064, Peoples R China

关键词： glucagon receptor; cDNA sequence; variation sites; diabetes; Bama minipig

期刊名称：CANADIAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：1.015； 五年影响因子：1.094 ）

ISSN： 0008-3984

年卷期： 2020 年 100 卷 3 期

页码：

收录情况： SCI

摘要： Recent studies have shown that the glucagon receptor (GCGR) plays an important role in the development of type 2 diabetes mellitus. Both pigs and humans exhibit significantly similar behaviors in their glucose and lipid metabolism. In this study, the obtained Bama minipig GCGR coding sequence was 1437 bp encoding 479 amino acids (AA), which demonstrated higher sequence homology with humans than other species. It showed the highest expression profile in the liver, followed by the lung and kidney. In addition, the three-dimensional structure analysis showed that the porcine GCGR protein also had a classic sevenfold transmembrane region and a stalk region at the N-terminus for ligand binding. The stalk region of GCGR possessed five AA variations. The ligand binding pocket of GCGR has one AA variation in the key region, none of which affected the glucagon binding verified by the crystal structure mutagenesis in humans. There was no variation found in the region of membrane anchoring, hydrophobic bond, salt bridge, and hydrogen bond. However, the Gly40Ser mutation in mice resulted in major diseases, meaning that pigs are more suitable for the evaluation of GCGR-related drugs than mice.

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