miR-196a regulates the skin pigmentation of koi carp (Cyprinus carpioL.) by targeting transcription factormitfa
文献类型： 外文期刊
作者： Yin, Haoran 1 ; Luo, Mingkun 1 ; Luo, Wentao 1 ; Wang, Lanmei 2 ; Zhu, Wenbin 2 ; Fu, Jianjun 2 ; Dong, Zaijie 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Freshwater Fisheries Res Ctr, Minist Agr & Rural Affairs, Wuxi, Jiangsu, Peoples R China
关键词： koi carp; miR-196a; mitfa; pigment; skin colour
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期：
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play important roles in many biological processes. The function of miRNAs in skin colour differentiation and pigmentation has been reported in many animals. However, the researches about their molecular mechanisms of regulating the formation of different skin colour of koi carp (Cyprinus carpioL.) are deficient. In this study, we detected the temporal and spatial expression levels of ccr-miR-196a (hereinafter referred to as miR-196a) and then predicted its target mRNA ofmicrophthalmia-associated transcription factor a(mitfa), which is a key regulatory transcript factor in the melanogenesis pathway, with bioinformatics and dual-luciferase reporter methods. Furthermore, we silenced the miR-196a in vivo with an antagomir to detect whether the miR-196a would affect the expression levels ofmitfamRNA and its downstream genes includingtyrosinase(tyr), tyrosinase-related protein1 (tyrp1) anddopachrome tautomerase(dct). The results revealed thatmitfaand its downstream genes' mRNA expressions all significantly increased in the treatment group compared with the control group. We presumed that miR-196a might regulate the pigmentation of koi carp through targeting themitfa. The finding provided the fundamental information regarding miRNA-mRNA interaction on different skin colour pigmentation in koi carp.
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