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Characterization and expression of arginine kinase 2 from Macrobrachium nipponense in response to salinity stress

文献类型： 外文期刊

作者： Huang, Youhui 1 ; Wu, Donglei 1 ; Li, Yiming 1 ; Chen, Qiang 1 ; Zhao, Yunlong 1 ;

作者机构： 1.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China

2.Shanghai Acad Agr Sci, Shanghai 201403, Peoples R China

3.East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China

关键词： Macrobrachium nipponense; MnAK2; Oxidative stress

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2020 年 113 卷

页码：

收录情况： SCI

摘要： Salinity is a fundamental environmental factor in aquaculture, and arginine kinase (AK) plays imperative roles in innate immune feedback and stress resistance in invertebrates. In the current study, we cloned a full-length cDNA of arginine kinase 2 (MnAK2, GenBank number, MN149533) in Macrobrachium nipponense and analyzed its function through a salinity challenge using bioinformatic approaches. MnAK2 was expressed at the highest levels in hepatopancreas and muscle. Changes in the expression levels of MnAK2, enzymes involved in innate immunity, antioxidant enzymes, and antioxidant enzyme-related genes, and the glutathione and malondialdehyde contents were investigated after 6-week salinity treatment. The expression of MnAK2 gradually increased as salinity increased, and western blotting showed that MnAK2 was significantly upregulated in the 14 and 22 ppt salinity-treatment groups relative to the control group. The findings indicate that high salinity produces oxidative stress and that salinity below isotonic salinity might improve the antioxidant response in M. nipponense. MnAK2 may play a crucial role in the response to salinity stress in M. nipponense.

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