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The ferroxidase LPR5 functions in the maintenance of phosphate homeostasis and is required for normal growth and development of rice

文献类型： 外文期刊

作者： Ai, Hao 1 ; Cao, Yue 2 ; Jain, Ajay 3 ; Wang, Xiaowen 1 ; Hu, Zhi 1 ; Zhao, Gengmao 1 ; Hu, Siwen 1 ; Shen, Xing 1 ; Yan, Ya 1 ;

作者机构： 1.Nanjing Agr Univ, Minist Agr, State Key Lab Crop Genet & Germplasm Enhancement, Key Lab Plant Nutr & Fertilizat Low Middle Reache, Nanjing 210095, Peoples R China

2.Sun Yat Sen Univ, Guangdong Prov Key Lab Environm Pollut Control &, Sch Environm Sci & Engn, Guangzhou 510006, Peoples R China

3.Amity Univ Rajasthan, Amity Inst Biotechnol, Jaipur, Rajasthan, India

4.Nanjing Agr Univ, Landscape Architecture Dept, Coll Hort, Nanjing 210095, Peoples R China

5.Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA

6.Shanghai Acad Agr Sci, Inst Ecoenvironm & Plant Protect, Shanghai 201403, Peoples R China

关键词： Agronomic traits; ferroxidase activity; growth and development; Oryza sativa; LPR5; phosphate homeostasis; rice

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2020 年 71 卷 16 期

页码：

收录情况： SCI

摘要： Members of the Low Phosphate Root (LPR) family have been identified in rice (Oryza sativa) and expression analyses have been conducted. Here, we investigated the functions of one of the five members in rice, LPR5. qRT-PCR and promoter-GUS reporter analyses indicated that under Pi-sufficient conditions OsLPR5 was highly expressed in the roots, and specific expression occurred in the leaf collars and nodes, and its expression was increased under Pi-deficient conditions. In vitro analysis of the purified OsLPR5 protein showed that it exhibited ferroxidase activity. Overexpression of OsLPR5 triggered higher ferroxidase activity, and elevated concentrations of Fe(III) in the xylem sap and of total Fe in the roots and shoots. Transient expression of OsLPR5 in Nicotiana benthamiana provided evidence of its subcellular localization to the cell wall and endoplasmic reticulum. Knockout mutation in OsLPR5 by means of CRISPR-Cas9 resulted in adverse effects on Pi translocation, on the relative expression of Cis-NATOsPHO1;2, and on several morphological traits, including root development and yield potential. Our results indicate that ferroxidasedependent OsLPR5 has both a broad-spectrum influence on growth and development in rice as well as affecting a subset of physiological and molecular traits that govern Pi homeostasis.

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