The NLR Protein Encoded by the Resistance GeneTy-2Is Triggered by the Replication-Associated Protein Rep/C1 of Tomato Yellow Leaf Curl Virus
文献类型： 外文期刊
作者： Shen, Xuexue 1 ; Yan, Zhe 1 ; Wang, Xiaoxuan 3 ; Wang, Yinlei 4 ; Arens, Marjon 1 ; Du, Yongchen 3 ; Visser, Richard G 1 ;
作者机构： 1.Wageningen Univ & Res, Plant Breeding, Wageningen, Netherlands
2.Wageningen Univ & Res, Grad Sch Expt Plant Sci, Wageningen, Netherlands
3.Chinese Acad Agr Sci, Inst Vegetable & Flowers, Beijing, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing, Peoples R China
5.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing, Peoples R China
6.Wageningen Univ & Res, Virol Lab, Wageningen, Netherlands
关键词： TYLCV; Ty-2; cloning; NLR gene; Rep; C1; avirulence factor
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）
ISSN： 1664-462X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： The whitefly-transmitted tomato yellow leaf curl virus (TYLCV) is one of the most destructive viral pathogens of cultivated tomato. To combat TYLCV, resistance geneTy-2has been introduced into cultivated tomato (Solanum lycopersicum) from wild tomato speciesSolanum habrochaitesby interspecific crossing. Introgression lines withTy-2contain a large inversion compared withS. lycopersicum, which causes severe suppression of recombination and has hampered the cloning ofTy-2so far. Here, we report the fine-mapping and cloning ofTy-2using crosses between aTy-2introgression line and several susceptibleS. habrochaitesaccessions.Ty-2was shown to encode a nucleotide-binding leucine-rich repeat (NLR) protein. For breeding purposes, a highly specific DNA marker tightly linked to theTy-2gene was developed permitting marker-assisted selection. The resistance mediated byTy-2was effective against the Israel strain of TYLCV (TYLCV-IL) and tomato yellow leaf curl virus-[China : Shanghai2] (TYLCV-[CN : SH2]), but not against tomato yellow leaf curl Sardinia virus (TYLCSV) and leafhopper-transmitted beet curly top virus (BCTV). By co-infiltration experiments we showed that transient expression of the Rep/C1 protein of TYLCV, but not of TYLCSV triggered a hypersensitive response (HR) inNicotiana benthamianaplants co-expressing theTy-2gene. Our results indicate that theRep/C1gene of TYLCV-IL presents the avirulence determinant ofTy-2-mediated resistance.
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