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Microbispora cellulosiformanssp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region

文献类型： 外文期刊

作者： Gong, Xiujie 1 ; Xiang, Wensheng 3 ; Cao, Xu 4 ; Yu, Yang 2 ; Hao, Yubo 2 ; Li, Liang 2 ; Wang, Qiuju 5 ; Zou, Hongtao 1 ; Q 1 ;

作者机构： 1.Shenyang Agr Univ, Coll Land & Environm, 120 DongLing Rd, Shenyang 110866, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Farming & Cultivat, 368 Xuefu Rd, Harbin 150086, Peoples R China

3.Northeast Agr Univ, Key Lab Agr Biol Funct Gene, Heilongjiang Prov Educ Comm, 59 Mucai St, Harbin 150030, Peoples R China

4.Heilongjiang Acad Sci, Inst Microbiol, 68 Zhaolin St, Harbin 150086, Peoples R China

5.Heilongjiang Acad Agr Sci, Inst Soil Fertilizer & Environm Resources, 368 Xuefu Rd, Harbin 150086, Peoples R China

关键词： Microbispora cellulosiformanssp; nov; Polyphasic taxonomy; 16S rRNA gene; Cellulase activity

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期：

页码：

收录情况： SCI

摘要： A novel cellulase-producing actinomycete strain Gxj-6(T), isolated from soil in the cold region (Heihe city, Heilongjiang province, the northernmost part of China), subjected to a taxonomic study using a polyphasic approach. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain Gxj-6(T)fell within the clade comprising the type strains of species of the genusMicrobispora. 16S rRNA gene sequence similarity studies exhibited that species Gxj-6(T)was most closely related toMicrobispora bryophytorumNEAU-TX2-2(T)(99.45%),Microbispora fuscaNEAU-HEGS1-5(T)(99.41%),Microbispora camponoti2C-HV3(T)(99.31%) andMicrobispora roseasubsp.roseaJCM 3006(T)(98.68%). Organism Gxj-6(T)contained MK-9(H-2) as the predominant ubiquinone and C(18:0)10-methyl as the major fatty acid. The major polar lipids of culture Gxj-6(T)were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, three unidentified phospholipids, two unidentified glycolipids and two unidentified lipids. The DNA G+C content of strain Gxj-6(T)was 71.25 mol%. The morphological and chemotaxonomic properties of the strain are also consistent with those members of the genusMicrobispora. Combinated with the lower DNA-DNA relatedness values, phenotypic properties, physiology and biochemistry distinctiveness with other recognized species strains, revealed that strain Gxj-6(T)is separated from other phylogenetically closely species of the genusMicrobispora. Therefore, strain Gxj-6(T)is considered to represent a novel species of the genusMicrobispora, for which the nameMicrobispora cellulosiformanssp. nov. is proposed. The type strain is Gxj-6(T)(= CGMCC 4.7605(T) = DSM 109712(T)).

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