A novel Rap-Phr system inBacillus velezensisNAU-B3 regulates surfactin production and sporulation via interaction with ComA
文献类型： 外文期刊
作者： Liang, Zhao 1 ; Qiao, Jun-Qing 3 ; Li, Ping-Ping 1 ; Zhang, Lu-Lu 1 ; Qiao, Zi-Xuan 1 ; Lin, Ling 1 ; Yu, Chen-Jie 1 ; Ya 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Jiangsu, Peoples R China
2.Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
4.Nordreet UG Greifswald, Greifswald, Germany
5.Inst Marine Biotechnol eV IMaB, Greifswald, Germany
关键词： Quorum sensing system; Rap-Phr system; Bacillus; Surfactin; Sporulation
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）
ISSN： 0175-7598
年卷期：
页码：
收录情况： SCI
摘要： Several quorum sensing systems occurring inBacillus subtilis, e.g. Rap-Phr systems, were reported to interact with major regulatory proteins, such as ComA, DegU, and Spo0A, in order to regulate competence, sporulation, and synthesis of secondary metabolites. In this study, we characterized a novel Rap-Phr system, RapA4-PhrA4, inBacillus velezensisNAU-B3. We found that therapA4andphrA4genes were co-transcribed in NAU-B3. WhenrapA4was expressed in the heterologous hostBacillus subtilisOKB105, surfactin production and sporulation were severely inhibited. However, when thephrA4was co-expressed, the RapA4 activity was inhibited. The transcription of the surfactin synthetasesrfAgene and sporulation-related genes were also regulated by the RapA4-PhrA4 system. In vitro results obtained from electrophoretic mobility shift assay (EMSA) proved that RapA4 inhibits ComA binding to the promoter of thesrfAoperon, and the PhrA4 pentapeptide acts as anti-activator of RapA4. We also found that the F24 residue plays a key role in RapA4 function. This study indicated that the novel RapA4-PhrA4 system regulates the surfactin synthesis and sporulation via interaction with ComA, thereby supporting the bacterium to compete and to survive in a hostile environment.
- 相关文献
作者其他论文 更多>>
-
Research advances in the identification of regulatory mechanisms of surfactin production by Bacillus: a review
作者：Qiao, Junqing;Zhang, Rongsheng;Liu, Youzhou;Liu, Yongfeng;Borriss, Rainer;Sun, Kai;Chen, Xijun
关键词：Bacillus; Surfactin; Regulatory mechanism
-
Fusarium graminearum rapid alkalinization factor peptide negatively regulates plant immunity and cell growth via the FERONIA receptor kinase
作者：Wang, Yujie;Liu, Xin;Yuan, Bingqin;Chen, Xue;Zhao, Hanxi;Ali, Qurban;Zheng, Minghong;Tan, Zheng;Yao, Hemin;Zheng, Shuqing;Wu, Jingni;Xu, Jianhong;Shi, Jianrong;Wu, Huijun;Gao, Xuewen;Gu, Qin;Liu, Xin;Xu, Jianhong;Shi, Jianrong
关键词：Fusarium graminearum; rapid alkalinization factor; receptor kinase FERONIA; host immunity; plant growth
-
A Scalable and Robust Chloroplast Genotyping Solution: Development and Application of SNP and InDel Markers in the Maize Chloroplast Genome
作者：Wang, Rui;Yang, Yang;Tian, Hongli;Yi, Hongmei;Xu, Liwen;Ge, Jianrong;Zhao, Yikun;Wang, Lu;Wang, Fengge;Lv, Yuanda;Zhou, Shiliang
关键词：chloroplast; SNP; InDel; high throughput; genotyping; maize
-
Matrine Ameliorates DSS-Induced Colitis by Suppressing Inflammation, Modulating Oxidative Stress and Remodeling the Gut Microbiota
作者：Mao, Ningning;Yu, Yaming;He, Jin;Yang, Yang;Liu, Zhenguang;Wang, Deyun;Mao, Ningning;Yu, Yaming;He, Jin;Yang, Yang;Liu, Zhenguang;Wang, Deyun;Lu, Yu
关键词：DSS; matrine; ulcerative colitis; Treg/Th17; gut microbiota
-
Plant-Microbes Interaction: Exploring the Impact of Cold-Tolerant Bacillus Strains RJGP41 and GBAC46 Volatiles on Tomato Growth Promotion through Different Mechanisms
作者：Khan, Abdur Rashid;Ali, Qurban;Ayaz, Muhammad;Bilal, Muhammad Saqib;Gu, Qin;Wu, Huijun;Gao, Xuewen;Sheikh, Taha Majid Mahmood
关键词：Bacillus strains; tomato plants; volatile organic compounds; GC-MS analysis; PGPR; antioxidant enzymes
-
Impact of Quorum Sensing Signal N-3-Oxododecanoyl Homoserine Lactone on Intestinal Porcine Enterocyte Cell
作者：Yang, Yang;Wang, Congrong;Liu, Yun;Ma, Xinyan;Xia, Pengpeng;Duan, Qiangde;Zhu, Guoqiang;Yang, Yang;Wang, Congrong;Liu, Yun;Ma, Xinyan;Xia, Pengpeng;Duan, Qiangde;Zhu, Guoqiang;Yang, Yang;Wang, Congrong;Liu, Yun;Ma, Xinyan;Xia, Pengpeng;Duan, Qiangde;Zhu, Guoqiang;Zhou, Mingxu;Zhou, Mingxu
关键词：Quorum Sensing; 3OC12-HSL; N-3-Oxododecanoyl Homoserine Lactone (OdDHL); Hexanoyl-L-Homoserine Lactone (C6-HSL); Epithelial Cells in the Small Intestine of Piglets; Biochemicals.
-
Impact of Quorum Sensing Signal N-3-Oxododecanoyl Homoserine Lactone on Intestinal Porcine Enterocyte Cell
作者：Yang, Yang;Wang, Congrong;Liu, Yun;Ma, Xinyan;Xia, Pengpeng;Duan, Qiangde;Zhu, Guoqiang;Yang, Yang;Wang, Congrong;Liu, Yun;Ma, Xinyan;Xia, Pengpeng;Duan, Qiangde;Zhu, Guoqiang;Yang, Yang;Wang, Congrong;Liu, Yun;Ma, Xinyan;Xia, Pengpeng;Duan, Qiangde;Zhu, Guoqiang;Zhou, Mingxu;Zhou, Mingxu
关键词：Quorum Sensing; 3OC12-HSL; N-3-Oxododecanoyl Homoserine Lactone (OdDHL); Hexanoyl-L-Homoserine Lactone (C6-HSL); Epithelial Cells in the Small Intestine of Piglets; Biochemicals
