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Identification of Genes Involved in Antifungal Activity of Burkholderia seminalis Against Rhizoctonia solani Using Tn5 Transposon Mutation Method

文献类型： 外文期刊

作者： Zhang, Muchen 1 ; Wang, Xiaoxuan 1 ; Ahmed, Temoor 1 ; Liu, Mengju 1 ; Wu, Zhifeng 1 ; Luo, Jinyan 3 ; Tian, Ye 1 ; Jiang 1 ;

作者机构： 1.Zhejiang Univ, State Key Lab Rice Biol, Inst Biotechnol, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Minist Agr, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China

3.Shanghai Extens & Serv Ctr Agr Technol, Dept Plant Quarantine, Shanghai 201103, Peoples R China

4.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

关键词： antagonism; Tn5; Burkholderia seminalis; Rhizoctonia solani; rice sheath blight

期刊名称：PATHOGENS （ 影响因子：3.492； 五年影响因子：4.066 ）

ISSN：

年卷期： 2020 年 9 卷 10 期

页码：

收录情况： SCI

摘要： Rhizoctonia solani is the causative agent of rice sheath blight disease. In a previous study, we found that the growth of R. solani was inhibited by Burkholderia seminalis strain R456. Therefore, the present study was conducted to identify the genes involved in the antifungal activity of B. seminalis strain R456 by using a Tn5 transposon mutation method. Firstly, we constructed a random insertion transposon library of 997 mutants, out of which 11 mutants showed the defective antifungal activity against R. solani. Furthermore, the 10 antagonism-related genes were successfully identified based on analysis of the Tn5 transposon insertion site. Indeed, this result indicated that three mutants were inserted on an indigenous plasmid in which the same insertion site was observed in two mutants. In addition, the remaining eight mutants were inserted on different genes encoding glycosyl transferase, histone H1, nonribosomal peptide synthetase, methyltransferase, MnmG, sulfate export transporter, catalase/peroxidase HPI and CysD, respectively. Compared to the wild type, the 11 mutants showed a differential effect in bacteriological characteristics such as cell growth, biofilm formation and response to H2O2 stress, revealing the complexity of action mode of these antagonism-related genes. However, a significant reduction of cell motility was observed in the 11 mutants compared to the wild type. Therefore, it can be inferred that the antifungal mechanism of the 10 above-mentioned genes may be, at least partially, due to the weakness of cell motility. Overall, the result of this study will be helpful for us to understand the biocontrol mechanism of this bacterium.

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