文献类型： 外文期刊

作者： Gong, Xiujie ¹ ; Zou, Hongtao ¹ ; Qian, Chunrong ² ; Yu, Yang ² ; Hao, Yubo ² ; Li, Liang ² ; Wang, Qiuju ³ ; Jiang, Yubo ² ;

作者机构： 1.Shenyang Agr Univ, Coll Land & Environm, 120 DongLing Rd, Shenyang 110866, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Farming & Cultivat, 368 Xuefu Rd, Harbin 150086, Peoples R China

3.Heilongjiang Acad Agr Sci, Inst Soil Fertilizer & Environm Resources, 368 Xuefu Rd, Harbin 150086, Peoples R China

4.Heilongjiang Acad Agr Sci, Inst Biotechnol, 368 Xuefu Rd, Harbin 150086, Peoples R China

关键词： Corn straw; In situ degrading bacteria; Strain identification; Flora construction; Degradation efficiency

期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）

ISSN： 1590-4261

年卷期： 2020 年 70 卷 1 期

页码：

收录情况： SCI

摘要： Purpose The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization. Methods According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Result The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain Streptomyces sp. G1(T) has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain Streptomyces sp. G2(T) has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain Streptomyces sp. G3(T) to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 degrees C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Conclusion Streptomyces sp. G1(T), Streptomyces sp. G2(T), and Streptomyces sp. G3(T) screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources.