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Prevalence, Genotypic and Phenotypic Characterization and Antibiotic Resistance Profile of Clostridium perfringens Type A and D Isolated from Feces of Sheep (Ovis aries) and Goats (Capra hircus) in Punjab, Pakistan

文献类型： 外文期刊

作者： Mohiuddin, Mudassar 1 ; Iqbal, Zahid 3 ; Siddique, Abubakar 4 ; Liao, Shenquan 1 ; Salamat, Muhammad Khalid Faro 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Key Lab Livestock Dis Prevent Guangdong Prov,Inst, Minist Educ,Maoming Branch,Guangdong Lab Lingnan, Guangzhou 510640, Peoples R China

2.Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Dept Microbiol, Bahawalpur 63100, Pakistan

3.Swat Med Coll, Dept Pharmacol, Marghzar Rd, Saidu Sharif 19200, Swat, Pakistan

4.Natl Univ Sci & Technol NUST, Atta Ur Rahman Sch Appl Biosci ASAB, H-12, Islamabad 44000, Pakistan

5.Univ Edinburgh, Roslin Inst, Easter Bush EH25 9RG, Midlothian, Scotland

6.Virtual Univ Pakistan, Dept Biotechnol, 1 Davis Rd, Lahore 54000, Pakistan

关键词： C; perfringens; antimicrobial susceptibility; sheep; goats; toxinotyping; multiplex PCR

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2020 年 12 卷 10 期

页码：

收录情况： SCI

摘要： Clostridium perfringens poses a serious threat to small ruminants by causing moderate to severe enterotoxaemia. Due to its ability to produce a wide arsenal of toxins, it is ranked among the most prevalent and important pathogens in livestock. This study focused on the molecular characterization of different Clostridium perfringens types along with their antimicrobial resistance profile. An overall higher prevalence of C. perfringens (46.1%) was detected based on mPCR among sheep and goats (healthy and diseased) in the Punjab province, Pakistan. The majority of the isolates were characterized as type A (82%), followed by type D (18%). Among the isolates from diseased sheep and goats, 27% were positive for cpa, 49% for cpa and cpb2, 9% for cpa and etx, 15% for cpa, cpb2 and etx. In the case of isolates from healthy sheep and goats, 59% were positive for cpa, 34% for cpb2 and cpa, 4% for cpa and etx, and 3% for cpa, cpb2 and etx. The prevalence of the beta2 toxin gene in the diseased sheep and goat population was 64% as compared to 37% in healthy animals. All 184 isolates (100%) were sensitive to rifampin and ceftiofur; the majority (57%) was sensitive to teicoplanin, chloramphenicol, amoxicillin, linezolid and enrofloxacin. A lower proportion of isolates (43%) were sensitive to ciprofloxacin and only 14% were susceptible to erythromycin. The findings of this study highlight the higher prevalence of C. perfringens in small ruminants and indicate that detailed pathogenesis studies are necessary to understand the explicit role of various toxins in causing enteric infections in sheep and goats including how they might be exploited to develop vaccines against these diseases.

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