文献类型： 外文期刊

作者： Zheng, Luzhe ¹ ; Wang, Zhanzhan ¹ ; Zhang, Bo ¹ ; Yan, Lulu ³ ; Wang, Pengfei ³ ; Zhao, Chao ¹ ; Wang, Jun ³ ; Wang, Yun ³ ; Lin, Heizhao ⁴ ; Qiu, Lihua ¹ ; Zhou, Chuanpeng ¹ ;

作者机构： 1.Minist Agr & Rural Affairs, South China Sea Fisheries Res Inst, Key Lab Fishery Resources Dev & Utilizat South Chi, Guangzhou 510300, Peoples R China

2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

3.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Guangzhou 510300, Peoples R China

4.Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572426, Peoples R China

5.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518121, Peoples R China

关键词： Intestinal barrier; Intestinal immunity; Inflammatory; Intestinal microbiota; Rhodiola rosea L.; Lateolabrax maculatus

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 146 卷

页码：

收录情况： SCI

摘要： A 56-d feeding trial was conducted to evaluate the influences of Rhodiola rosea L. on digestive enzyme activities, intestinal barrier, inflammatory response, and microbiota dysbiosis in Lateolabrax maculatus juveniles (9.37 +/- 0.03 g) fed with high -carbohydrate diets. Six diets were designed: a control diet (20% corn starch, Control), highcarbohydrate diet (30% corn starch, HC1), and four high -carbohydrate diets supplemented with Rhodiola rosea L. at 30, 60, 90 and 120 mg/kg (HC2, HC3, HC4 and HC5, respectively). Compared with the control group, the HC1 diet remarkably increased alpha-amylase, lipase, and chymotrypsin activities in the intestine (p < 0.05), as well as the mRNA levels of Claudin-15, NF -KB, TNF-alpha, IL-1 beta, and IL -8 (p < 0.05) and the relative abundance of Proteobacteria and Photobacterium in the intestine, which belong to the phylum and genus level, respectively. But the opposite trend was found in muscular thickness and villus lengths (p < 0.05), the mRNA levels of Occludin, ZO-1, and TGF beta (p < 0.05), at the level of phylum and genus level in the HC1 group, and the relative abundance of Firmicutes, Bacteroidetes, and Bacillus in the intestine compared with the control group. Intestinal chymotrypsin activity was significantly higher in the HC3 group and intestinal muscular thickness and villus lengths were also significantly higher in the HC2, HC3, HC4, and HC5 groups compared to the HC1 group (p < 0.05). In addition, Occludin mRNA expression in the intestine was significantly increased in the HC2, HC4, and HC5 groups compared to the HC1 group. ZO-1 and TGF-beta mRNA expression in the intestine were significantly increased in the HC2, HC3, HC4, and HC5 groups compared to the HC1 group (p < 0.05). At the phylum level, the relative abundance of Firmicutes and Bacteroidetes was higher in the intestine in the HC2, HC3, HC4, and HC5 groups than that in the HC1 group. On the contrary, intestinal lipase and chymotrypsin activities were significantly decreased in the HC2 group compared to the HC1 group, respectively (p < 0.05). The Claudin-15, NF -KB, TNF-alpha, IL-1 beta, and IL -8 mRNA expression in the intestine were significantly decreased in the HC2, HC3, HC4, and HC5 groups compared to the HC1 group (p < 0.05). Besides, at the genus level, compared to the HC1 group, the relative abundance of Photobacterium in the intestine and the diversity of the intestinal microbiota in the HC2, HC3, HC4, and HC5 groups were all decreased. In conclusion, these results demonstrated that the addition of Rhodiola rosea L. in high -carbohydrate diets can improve intestinal digestive enzyme activities, inflammatory response and intestinal barrier -related gene expression, and microbiota dysbiosis in L. maculatus. The suitable supplemental level of Rhodiola rosea L. in high -carbohydrate diets of L. maculatus is 60 mg/kg.