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Arginine Promotes the Expression of Aquaporin-3 and Water Transport in Porcine Trophectoderm Cells Through NO- and cAMP-Dependent Mechanisms

文献类型： 外文期刊

作者： Zhu, Cui 1 ; Ye, Jinling 2 ; Bai, Yinshan 1 ; Hu, Shengdi 3 ; Tan, Chengquan 3 ; Bazer, Fuller W. 3 ; Johnson, Gregory A. 4 ; Jiang, Zongyong 2 ; Wu, Guoyao 3 ;

作者机构： 1.Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China

3.Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA

4.Texas A&M Univ, Dept Integrat Biosci, College Stn, TX 77843 USA

关键词： arginine; aquaporin-3; pig; placenta; pregnancy; cAMP pathway

期刊名称：FRONTIERS IN BIOSCIENCE-LANDMARK （ 影响因子：3.115； 五年影响因子：4.315 ）

ISSN： 2768-6701

年卷期： 2022 年 27 卷 3 期

页码：

收录情况： SCI

摘要： Background: Dietary supplementation with L-arginine (Arg) has been shown to increase the volume of fetal fluids in gestating swine. Aquaporins (AQPs), known as water channel proteins, are essential for embryonic growth and development. It was not known if Arg mediates water transport through AQPs in porcine conceptus trophectoderm (pTr2) cells. Methods: pTr2 cells derived from pregnant gilts on day 12 of gestation were cultured in customized Arg-free Dulbecco's modified Eagle's Ham medium (DMEM) supplemented with either 0.00, 0.25, or 0.50 mM Arg. Results: Arg treatment increased water transport and the expression of AQP3, which was abundantly expressed in pTr2 cells at both the mRNA and protein levels. Arg also increased the expression of iNOS and the synthesis of nitric oxide (NO) in pTr2 cells. The presence of N omega-nitro-L-arginine methyl ester hydrochloride (L-NAME; an inhibitor of NO synthase) significantly attenuated the Arg-induced expression of AQP3. Furthermore, 0.50 mM Arg increased the concentrations of cAMP and the abundances of phosphorylated cAMP-dependent protein kinase A (PKA), phosphorylated PKA oi/beta/gamma, and phosphorylated CREB. These effects of Arg were mimicked by Forskolin (a cell-permeable activator of adenylyl cyclase), but inhibited by H-89 (an inhibitor of cAMP-dependent protein kinase). Conclusions: The results of this study demonstrate that Arg regulates AQP3 expression and promotes water transport in pTr2 cells through NO- and cAMP-dependent signaling pathways.

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