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Direct in vitro organogenesis from sprouted seeds of a highly economical and ecological valued tree, Korean pine

文献类型： 外文期刊

作者： Liang, Yan 1 ; Bai, Xue 1 ; Xu, Xin 1 ; Xu, Hongguo 1 ; Wang, Jing 3 ; Pan, Peng 1 ;

作者机构： 1.Qiqihar Univ, Sch Life Sci & A&F, Qiqihar 161006, Peoples R China

2.Prov Key Lab Resistance Gene Engn & Protect Biodi, Qiqihar 161006, Peoples R China

3.Heilongjiang Acad Agr Sci, Qual & Safety Inst Agr Prod, Harbin 150086, Peoples R China

关键词： Pinus koraiensis; In vitro propagation; Shoot organogenesis; Plant growth regulators

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期：

页码：

收录情况： SCI

摘要： Korean pine (Pinus koraiensis Sieb. et Zucc.) is a critical coniferous species ecologically. In addition, its fruits have numerous nutraceutical properties and its wood is high quality timber. However, this plant has a low propagation rate and is under risk of extinction. To accelerate breeding of the excellent seedlings and selection from Korean pine, determining methods to increase the number of Korean pine trees successfully is imperative. In this present study, using sprouted seeds as the initial explants, the de novo organogenesis system for this coniferous species has been successfully developed. After 30 days of incubation on Gupta and Durzan (DCR) medium containing 2 mg L-1 kinetin (KT) and 0.5 mg L-1 thidiazuron (TDZ), 92.67% of explants produced direct buds with an average of about 15 buds per explant. We confirmed the organogenic regeneration pattern by a scanning electron microscopy (SEM). For bud elongation after 60 days of culture, we obtained the highest mean length of 34.99 mm on DCR basal media supplemented with 6-benzyladenine (6-BA, 0.2 mg L-1), 1-naphthaleneacetic acid (NAA, 0.1 mg L-1), and activated charcoal (AC, 1 g L-1). The highest rooting rates of 20.74 and 21.48% were obtained within two months of culturing on 1/2 DCR medium (halved major elements) supplemented with 0.05 mg center dot L-1 NAA and 0.5 or 1 mg center dot L-1 indole-3-butyric acid (IBA), respectively. Rooted shoots showed a survival rate of 90.28% after acclimatization in the substrate consisting of perlite, peat, and vermiculite (1:1:1). This protocol is a successful and efficient biotechnological approach to the micropropagation of Korean pine, and the results will be helpful to the clonal propagation and conservation of Korean pine. Key message This study developed a useful protocol for multiplying plantlets from sprouted seed explants of Korean pine, an economically and ecologically important coniferous species.

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