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A colorimetric and SERS dual-signal immunochromatography based on AuDTNB@PDA nanoparticles for the detection of triadimefon

文献类型： 外文期刊

作者： Dong, Sa 1 ; Zhu, Zixin 1 ; Shi, Qiuyun 1 ; Zhang, Yu 1 ; Wang, Yulong 2 ; Luo, Ruifeng 3 ; Feng, Jianguo 1 ;

作者机构： 1.Yangzhou Univ, Coll Plant Protect, Yangzhou 225009, Peoples R China

2.Minist Agr, Inst Food Safety & Nutr, State Key Lab Breeding Base, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing 210014, Peoples R China

3.Minist Agr, Xinjiang Acad Agr & Reclamat Sci, Food Qual Supervis & Testing Ctr, Shihezi 832000, Peoples R China

关键词： Triadimefon residue; Immunochromatographic strip; Colorimetric; SERS; Dual-signal

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.6； 五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2025 年 140 卷

页码：

收录情况： SCI

摘要： In this study, anti-TDF monoclonal antibody (mAb) was combined with Au-DTNB@PDA as SERS probe, and a colorimetric and SERS dual-signal immunochromatography was designed to detect TDF residues. In the most favorable condition, the minimum visual detection limit (vLOD) of the immunochromatographic strip is 7.8 ng/ mL, facilitating the qualitative identification of TDF. A strong linear correlation between the Raman signal intensity and TDF concentration was observed from 3.9 to 125 ng/mL. The linear model was y =-0.68x + 242.80 (R-2 = 0.98), with a limit of detection (LOD) at 2.45 ng/mL. The immunochromatographic strip demonstrates high specificity towards TDF, and has no obvious cross-reaction with the four structural analogizes of TDF. Finally, the immunochromatographic strip was used to detect TDF in cucumber and tobacco leaves. The recovery rate was 87.90-107.70 %, and RSD <6.18 %, suggesting that the immunochromatographic strip is suitable for real-sample detection with good accuracy.

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