Quantitative determination of prothioconazole in wheat grain, soybean, and pond water based on a polyclonal antibody
文献类型： 外文期刊
作者： Guo, Zhihui 1 ; Deng, Mingya 3 ; Fang, Wenwen 3 ; Zheng, Kang 3 ; Liao, Min 2 ; Wang, Yulong 4 ; Fang, Qingkui 1 ;
作者机构： 1.Anhui Agr Univ, Key Lab Agriprod Qual & Biosafety, Minist Educ, Hefei 230036, Peoples R China
2.Anhui Agr Univ, Joint Res Ctr Food Nutr & Hlth IHM, Hefei 230036, Peoples R China
3.Anhui Agr Univ, Sch Plant Protect, Anhui Prov Key Lab Integrated Pest Management Crop, Hefei 230036, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov,Minist Agr, Nanjing 210014, Peoples R China
5.Xinjiang Acad Agr & Reclamat Sci, Food Qual Supervis & Testing Ctr, Minist Agr, Shihezi, Xinjiang, Peoples R China
期刊名称：ANALYTICAL METHODS （ 影响因子：3.1； 五年影响因子：3.0 ）
ISSN： 1759-9660
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Prothioconazole and its metabolite are considered a potential threat to human health and environmental safety. Thus, the development of a sensitive and rapid detection method for prothioconazole is crucial to ensure the safety of agricultural products. In this study, a new hapten of prothioconazole was designed and synthesized, and a selective polyclonal antibody with high affinity against prothioconazole was produced, which was obtained from immunized New Zealand white rabbits. Based on the polyclonal antibody, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) were developed for detecting prothioconazole pesticides. Under optimized experimental conditions, the limit of quantification (LOQ) values for ic-CLEIA and ic-ELISA were 1.8 and 10.7 ng mL-1, respectively. The results demonstrated that the sensitivity (LOQ) achieved by ic-CLEIA was more than five times higher compared to that obtained with ic-ELISA. In addition, the recoveries obtained by adding standard prothioconazole to wheat grain, soybean, and pond water samples were in the range of 81.9 to 104.7% for ic-ELISA and 89.0 to 118.0% for ic-CLEIA. Prothioconazole and its metabolite are considered a potential threat to human health and environmental safety.
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