Unraveling the spoilage characteristics of refrigerated pork using high-throughput sequencing coupled with UHPLC-MS/MS-based non-targeted metabolomics
文献类型： 外文期刊
作者： Yi, Zhengkai 1 ; Xiao, Xingning 1 ; Cai, Wei 2 ; Ding, Zhaoyang 3 ; Ma, Jiele 1 ; Lv, Wentao 1 ; Yang, Hua 1 ; Xiao, Yingping 1 ; Wang, Wen 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, MOA Lab Qual & Safety Risk Assessment Agroprod Han, Hangzhou, Peoples R China
2.Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430048, Peoples R China
3.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
4.Marine Biomed Sci & Technol Innovat Platform Lin g, Shanghai 201306, Peoples R China
关键词： Refrigerated pork; Microbiome; Metabolomics; Spoilage bacteria; Spoilage biomarkers
期刊名称：FOOD CHEMISTRY （ 影响因子：8.5； 五年影响因子：8.2 ）
ISSN： 0308-8146
年卷期： 2024 年 460 卷
页码：
收录情况： SCI
摘要： The spoilage of refrigerated pork involves nutrient depletion and the production of spoilage metabolites by spoilage bacteria, yet the microbe-metabolite interactions during this process remain unclear. This study employed 16S rRNA high-throughput sequencing and non-targeted metabolomics based on ultra-highperformance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to reveal the core microbiota and metabolite profiles of pork during refrigeration. A total of 45 potential biomarkers were screened through random forest model analysis. Metabolic pathway analysis indicated that eleven pathways, including biogenic amine metabolism, pentose metabolism, purine metabolism, pyrimidine metabolism, phospholipid metabolism, and fatty acid degradation, were potential mechanisms of pork spoilage. Correlation analysis revealed nine metabolites-histamine, tyramine, tryptamine, D-gluconic acid, UDP-D-glucose, xanthine, glutamine, phosphatidylcholine, and hexadecanoic acid-as spoilage biomarkers, with Pseudomonas, Serratia, and Photobacterium playing significant roles. This study provides new insights into the changes in microbial and metabolic characteristics during the spoilage of refrigerated pork.
- 相关文献
作者其他论文 更多>>
-
Linking growth performance and carcass traits with enterotypes in Muscovy ducks
作者：Fan, Qian;Xu, Yini;Yang, Caimei;Lyu, Wentao;Fan, Qian;Xu, Yini;Xiao, Yingping;Lyu, Wentao;Yang, Hua
关键词：Enterotype; Ileal Microbiota; Muscovy Duck; 16S rRNA Gene Sequencing
-
Distribution and safety evaluation of deoxynivalenol and its derivatives throughout the wheat product processing chain
作者：Ji, Xiaofeng;He, Yeyu;Xiao, Yingping;Yang, Hua;Wang, Xiaodan;Liang, Ying;Yang, Weikang;Guo, Cheng;Xiong, Lina;Zhang, Jiahong
关键词：Mycotoxins; Wheat; Food chain; Processing; Food safety; Regulation
-
A Bioinspired Photosensitizer Performs Tumor Thermoresistance Reversion to Optimize the Atraumatic Mild-Hyperthermia Photothermal Therapy for Breast Cancer
作者：Li, Jie;Yang, Die;Yuan, Yan;Han, Xiao;Yue, Weiqing;Jiang, Jian;Fang, Zhijie;Lu, Xiaomei;Huang, Wei;Li, Jie;Yang, Die;Yuan, Yan;Han, Xiao;Yue, Weiqing;Jiang, Jian;Fang, Zhijie;Lu, Xiaomei;Huang, Wei;Li, Jie;Yang, Die;Yuan, Yan;Han, Xiao;Yue, Weiqing;Jiang, Jian;Fang, Zhijie;Lu, Xiaomei;Huang, Wei;Lyu, Wentao;Xiao, Yingping;Wang, Wen;Lu, Xiaomei;Huang, Wei;Huang, Wei
关键词：atraumatic breast cancer therapy; bioinspired photosensitizer; mild-hyerthermia photothermal therapy; photodynamic effect; tumor thermoresistance
-
Comprehensive identification and risk assessment of regulated and emerging mycotoxins in infant foods and related raw materials and risk management advice: A case study of an infant food company in China
作者：Ji, Xiaofeng;Xiao, Yingping;Wei, Wang;Lyu, Wentao;Wang, Xiaoli;Yang, Hua;Yang, Weikang;Yang, Hua
关键词：Infant food; Wheat; Cereals; Mycotoxins; Food safety; Risk management
-
Kill two birds with one stone: To reduce toxicity and enhance photodegradation of antibiotics by lead cesium bromide via tin doping
作者：Gao, Jingyang;Wu, Jianhao;Liu, Chunxi;Chen, Zhi;Li, Lan;Hu, Jinglei;Li, Chunyan;Liu, Yurong;Wang, Xu-Sheng;Wang, Wen
关键词：CsPbBr3 perovskite; Sn doped; Anti-solvent precipitation; Antibiotic disposal; Visible-light photodegradation
-
Novel mechanism by which extracellular vesicles derived from Lactobacillus murinus alleviates deoxynivalenol-induced intestinal barrier disruption
作者：Fan, Jinping;Zhang, Yuhan;Zuo, Minyu;Ding, Shixuan;Li, Jingjing;Feng, Shengkai;Tao, Shiyu;Xiao, Yingping
关键词：Deoxynivalenol; Lactobacillus murinus; Extracellular vesicles; Macrophage; Gut barrier
-
Co-exposure to sodium hypochlorite and cadmium induced locomotor behavior disorder by influencing neurotransmitter secretion and cardiac function in larval zebrafish
作者：Ma, Lingyan;Yang, Hua;Xiao, Xingning;Chen, Qu;Lv, Wentao;Wang, Wen;Xiao, Yingping;Xu, Ting;Jin, Yuanxiang
关键词：Sodium hypochlorite; Cadmium; Zebrafish larvae; Oxidative stress; Neurotransmitters; Myocardial contraction
