Development and evaluation of a semi-automatic single-step clean-up apparatus for rapid analysis of 18 antibiotics in fish samples
文献类型： 外文期刊
作者： Mu, Shuhe 1 ; Teng, Tengwang 1 ; Zhou, Ruidong 1 ; Liu, Huan 1 ; Sun, Huiwu 1 ; Li, Jincheng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Beijing, Peoples R China
2.Minist Agr, Key Lab Control Qual & Safety Aquat Prod, Beijing 100141, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China
5.China Key Lab Control Qual & Safety Agr, Beijing 100141, Peoples R China
关键词： Antibiotics; Single-step clean-up; Semi -automatic pre-treatment apparatus; HPLC-QTRAP-MS; MS; Food composition
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.52； 五年影响因子：4.942 ）
ISSN： 0889-1575
年卷期： 2022 年 113 卷
页码：
收录情况： SCI
摘要： Here we report a novel semi-automatic pre-treatment apparatus to fulfill a single-step clean-up aim for rapid quantification of 18 antibiotics in fish samples. Trimethoprim and 17 sulfonamides were extracted from fish samples by acetonitrile solution based on ultrasonic-assisted extraction. Cheap analytical filtration syringe (CAFS) clean-up column was used to fulfill single-step clean-up of the extract which was set on the developed semi-automatic clean-up apparatus. This semi-automatic clean-up apparatus is quite convenient for single-step purification by precisely controlling the flow rate of the extracts, in which up to 10 samples can be purified in parallel at one time. The time needed to clean sample was only about 2 min per 10 samples. Type of compatible purification column ranged from 1 mL (inner diameter x length, 5.6 x 55 mm) to 12 mL (inner diameter x length, 15.6 x 83 mm). The apparatus also did not need additional gas source such as nitrogen or air. After purification, the analytes were simultaneously separated and quantified by high-performance liquid chromatography/quadrupole-linear ion trap tandem mass spectrometry (HPLC-QTRAP-MS/MS). The limits of detections ranged from 0.5 to 1.0 mu g kg- 1, and the limits of quantifications ranged from 1.0 to 5.0 mu g kg- 1. All the recoveries ranged from 81.39% to 116.01%. Intra-day and inter-day relative standard deviations (RSDs) were lower than 14% for all analytes.
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