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Genome-Wide Identification, Sequence Alignment, and Transcription of Five Sex-Related Genes in Largemouth Bass (Micropterus Salmoides)

文献类型： 外文期刊

作者： Zhang, Xinhui 1 ; Ruan, Zhiqiang 2 ; Sun, Chengfei 3 ; Hu, Cancan 4 ; Huang, Yu 2 ; You, Xinxin 1 ; Wang, Xinwen 2 ; Xu, Junmin 2 ; Liu, Huan 1 ; Liu, Xin 1 ; Ye, Xing 3 ; Shi, Qiong 1 ;

作者机构： 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

2.BGI Marine, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ An, BGI Acad Marine Sci, Shenzhen 518083, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resources Applicat, Minist Agr & Rural Affairs, Guangzhou 510380, Guangdong, Peoples R China

4.BGI Marine, Aquat Breeding Ctr, Shenzhen 518081, Guangdong, Peoples R China

5.BGI Shenzhen, State Key Lab Agr Genom, Shenzhen 518083, Guangdong, Peoples R China

6.Shenzhen Univ, Coll Life Sci & Oceanog, Lab Aquat Genom, Shenzhen 518060, Guangdong, Peoples R China

关键词： largemouth bass; sex-related gene; transcriptome sequencing; gonadal development; differentially expressed genes

期刊名称：FRONTIERS IN BIOSCIENCE-LANDMARK （ 影响因子：3.1； 五年影响因子：3.9 ）

ISSN： 2768-6701

年卷期： 2024 年 29 卷 2 期

页码：

收录情况： SCI

摘要： Background: Largemouth bass (Micropterus Salmoides) is an economically important fish species in China. Most research has focused on its growth, disease resistance, and nutrition improvement. However, the sex-determining genes in largemouth bass are still unclear. The transforming growth factor-beta (TGF-beta) gene family, including amh, amhr2 and gsdf, plays an important role in the sex determination and differentiation of various fishes. These genes are potentially involved in sex determination in largemouth bass. Methods: We performed a systematic analysis of 5 sex-related genes (amh, amhr2, gsdf, cyp19a1, foxl2) in largemouth bass using sequence alignment, collinearity analysis, transcriptome, and quantitative real-time polymerase chain reaction (qRT-PCR). This included a detailed assessment of their sequences, gene structures, evolutionary traits, and gene transcription patterns in various tissues including gonads, and at different developmental stages. Results: Comparative genomics revealed that the 5 sex-related genes were highly conserved in various fish genomes. These genes did not replicate, mutate or lose in largemouth bass. However, some were duplicated (amh, amhr2 and gsdf ), mutated (gsdf) or lost (amhr2) in other fishes. Some genes (e.g., gsdf ) showed significant differences in genomic sequence between males and females, which may contribute to sex determination and sex differentiation in these fishes. qRT-PCR was applied to quantify transcription profiling of the 5 genes during gonadal development and in the adult largemouth bass. Interestingly, amh, amhr2 and gsdf were predominantly expressed in the testis, while cyp19a1 and foxl2 were mainly transcribed in the ovary. All 5 sex-related genes were differentially expressed in the testes and ovaries from the 56th day post-fertilization (dpf). We therefore speculate that male/female differentiation in the largemouth bass may begin at this critical time-point. Examination of the transcriptome data also allowed us to screen out several more sex-related candidate genes. Conclusions: Our results provide a valuable genetic resource for investigating the physiological functions of these 5 sex-related genes in sex determination and gonadal differentiation, as well as in the control of gonad stability in adult largemouth bass.

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