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PSG076 Promoter Shows Late Pollen-Specific Activity in Wheat (Triticum aestivum L.)

文献类型： 外文期刊

作者： Chen, Ling 1 ; Su, Peipei 2 ; Yang, Guangxiao 2 ; He, Guangyuan 2 ; Gao, Chunbao 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Hubei Engn & Technol Res Ctr Wheat, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Hubei Acad Agr Sci,Inst Food Crops,Key Lab Crop Mo, Wuhan 430064, Hubei, Peoples R China

2.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Key Lab Mol Biophys, Genet Engn Int Cooperat Base Chinese,Minist Sci &, Wuhan 430074, Peoples R China

3.Yichun Univ, Coll Life Sci & Resource Environm, Yichun 336000, Peoples R China

关键词： Wheat; Pollen-specific promoter; PSG076 gene; GUS; Genetic transformation

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：2.1； 五年影响因子：2.0 ）

ISSN： 0735-9640

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Pollen, as the male gametophyte of plants, plays a critical role in plant sexual reproduction. Investigating pollen-specific promoters not only helps to understand the regulatory mechanisms of pollen development but also provides tissue-specific promoters for plant genetic engineering. Previously, wheat promoter PSG076 was found to confer pollen-specific activity in tobacco. To determine the tissue-specific activity of PSG076 in wheat, a 1.4-kb promoter fragment was fused with the & beta;-glucuronidase (GUS) reporter gene and stably introduced into wheat via particle bombardment. Histochemical analysis in T-1 progeny plants showed that the activity of PSG076 promoter was detected specifically in mature pollen and pollen tube. No GUS activity was found in other floral and vegetable tissues. Weak GUS staining was also visible in pollen at 45 days post anthesis (DPA). Further analysis showed that GUS activity was found weakly in middle tricellular pollen grains and increased rapidly as pollen matured. These results indicated that PSG076 promoter has strong activity specifically at late pollen development stage and can be utilized in genetic engineering investigations for creating male sterility in wheat and other plant species for hybrid seed production.

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