您好，欢迎访问海南省农业科学院 机构知识库！
海南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Knockdown of the ZcVgR Gene Alters the Expression of Genes Related to Reproduction and Lifespan in Zeugodacus cucurbitae (Coquillett) Under Extreme Heat Conditions

文献类型： 外文期刊

作者： Lian, Yuyang 1 ; Zhang, Mengjie 2 ; Yang, Shuyan 1 ; Peng, Sihua 1 ; Wang, Aqiang 1 ; Jia, Jingjing 4 ; Feng, Xuejie 5 ; Wu, Qianxing 1 ; Yang, Xiaofeng 1 ; Zhou, Shihao 1 ;

作者机构： 1.Hainan Univ, Sanya Nanfan Res Inst, Sanya, Peoples R China

2.Chongqing Univ, Sch Med, Chongqing, Peoples R China

3.Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing, Peoples R China

4.Hainan Acad Agr Sci, Res Ctr Qual Safety & Stand Agr Prod, Key Lab Plant Dis & Pest Control Hainan Prov, Res Ctr Qual Safety & Stand Agr Prod,Inst Plant Pr, Haikou, Peoples R China

5.Key Lab Trop Fruit Tree Biol Hainan Prov, Haikou, Peoples R China

关键词： lifespan; proteome; reproduction; transcriptome; ZcVgR gene; Zeugodacus cucurbitae (Coquillett)

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.9； 五年影响因子：2.2 ）

ISSN： 0739-4462

年卷期： 2024 年 117 卷 4 期

页码：

收录情况： SCI

摘要： Zeugodacus cucurbitae (Coquillett) is an important migratory vegetable pest. Previous research has demonstrated that short-term high temperatures induce differential expression of the vitellogenin receptor (ZcVgR) gene, reducing the number of eggs laid and the lifespan of female Z. cucurbitae. In this paper, we used Tandem Mass Tags (TMT) quantitative proteomics and Illumina high-throughput sequencing to determine the proteomic and transcriptomic information of female Z. cucurbitae after siRNA-mediated silencing of the target gene (ZcVgR) to gain a comprehensive understanding of the molecular mechanism of this gene in the regulation of reproduction and lifespan. The findings demonstrated that following the target gene's silencing, the ZcVgR gene's transcriptional expression was significantly downregulated, and there was no significant difference in protein level. The transcriptome and proteome had a low correlation; when the ZcVgR gene was silenced, vitellogenin-1 (ZcVg1), juvenile hormone epoxide hydrolase (JHEH), troponin C (TnC), heat shock protein 70 (HSP70), and other related genes were downregulated at the transcriptional level. By silencing the ZcVgR gene, transcriptionally level immune-related pathways were activated and energy metabolism-related pathways were inhibited; protein-level glycometabolism and phagosome pathways were activated, while phototransduction-fly and autophagy-animal pathways were inhibited. The findings of this study might offer a theoretical foundation for integrated management of Z. cucurbitae in the summertime.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：海南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台