Bicistronic operon YhaO-YhaM contributes to antibiotic resistance and virulence of pathogen Edwardsiella piscicida
文献类型： 外文期刊
作者： Wang, Duo 1 ; Gong, Chunguang 1 ; Gu, Hanjie 2 ; Huang, Huiqin 2 ; Xian, Jianan 2 ; Hu, Yonghua 1 ;
作者机构： 1.Hebei Agr Univ, Ocean Coll, Qinhuangdao 066000, Hebei, Peoples R China
2.CATAS, Inst Trop Biosci & Biotechnol, Hainan Acad Trop Agr Resource, Haikou 571101, Hainan, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Hainan Prov Key Lab Funct Components Res & Utiliz, Haikou 571101, Hainan, Peoples R China
关键词： Edwardsiella piscicida; YhaO-YhaM; Antibiotic resistance; Virulence
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2021 年 541 卷
页码：
收录情况： SCI
摘要： Edwardsiella piscicida is a leading pathogen that threats seawater and fresh aquaculture, in which the emergence and prevalence of bacterial resistance to antibiotics is a tricky problem. Currently, the pathogenesis of E. piscicida remains unclear, some new pathogenic factors need to be identified. Serine permease and dehydratase exist ubiquitously in microorganisms and participate in pathogenicity, but their functions in E. piscicida remain unknown. In this study, a serine permease YhaO and serine dehydratase YhaM were characterized in E. piscicida. yhaOEp and yhaMEp are expressed in the form of a bicistronic operon. YhaOEp possesses multiple transmembrane structures and YhaMEp is predicted to be located in the cytoplasm. To explore the biological roles of yhaOEp and yhaMEp, markerless in-frame mutant strains, TX01AyhaO and TX01AyhaM, were constructed, respectively. TX01, TX01AyhaO, and TX01AyhaM exhibited similar growth under normal and stress conditions, which indicates yhaOEp and yhaMEp are irrelevant to adversity. However, TX01AyhaO and TX01AyhaM displayed markedly reduced resistance against antibiotics, compared to TX01. Further studies indicated that the yhaOEp mutation damaged E. piscicida's ability to maintain membrane integrity and biofilm formation, decreased its adhesion and invasion to non-phagocytic FG cells, weakened the survival and proliferation in macrophage cells, and decreased bacterial dissemination in fish tissues. Complementary strain TX01AyhaOC restored these phenotypes of TX01AyhaO. The inactivation of yhaMEp has no effect on bacterial pathogenicity to FG cells and macrophage cells, but in vivo experiment showed that TX01AyhaM exhibited attenuated capability of bacterial dissemination in fish tissues, compared to TX01. Taken together, our findings demonstrate for the first time that YhaO-YhaM is requisite for full pathogenicity of E. piscicida to host cells and host tissues, and provide new insights into the pathogenesis of E. piscicida.
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